ULTRASTRUCTURAL-LOCALIZATION OF PEMPHIGUS VULGARIS AND PEMPHIGUS FOLIACEUS ANTIGENS IN CULTURED HUMAN SQUAMOUS CARCINOMA-CELLS
ULTRASTRUCTURAL-LOCALIZATION OF PEMPHIGUS VULGARIS AND PEMPHIGUS FOLIACEUS ANTIGENS IN CULTURED HUMAN SQUAMOUS CARCINOMA-CELLS
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DOI：
10.1111/j.1365-2133.1991.tb14746.x
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发表时间：
1991-09-01
影响因子：
10.3
通讯作者：
NISHIKAWA, T
中科院分区：
文献类型：
--
作者：
AKIYAMA, M;HASHIMOTO, T;NISHIKAWA, T
用免疫金电镜观察了寻常型天疱疮（PV）和落叶型天疱疮（PF）抗原在培养的人鳞癌细胞中的超微结构定位。PV和PF自身抗体都仅与桥粒结构的细胞外部分结合。在37 ℃孵育后，在培养的细胞内观察到PV抗原-抗体复合物。当细胞培养在低Ca ~（2+）浓度的培养基中时，PV和PF抗原表达显著降低。
The ultrastructural localization of the pemphigus vulgaris (PV) and pemphigus foliaceus (PF) antigens in cultured human squamous carcinoma cells was observed using immunogold electron microscopy. Both the PV and PF autoantibodies bound only to the extracellular portion of the desmosomal structures. After incubation at 37-degrees-C, the PV antigen-antibody complexes were observed within the cultured cells. PV and PF antigen expression was markedly reduced when the cells were cultured in medium with a low Ca2+ concentration.