Substantial high-affinity methanotroph populations in Andisols effect high rates of atmospheric methane oxidation.
Substantial high-affinity methanotroph populations in Andisols effect high rates of atmospheric methane oxidation.
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DOI：
10.1111/j.1758-2229.2009.00071.x
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发表时间：
2009-10
影响因子：
3.3
通讯作者：
P. Maxfield;E. Hornibrook;R. Evershed
中科院分区：
文献类型：
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作者：
P. Maxfield;E. Hornibrook;R. Evershed
通过时间序列 (13) C-磷脂脂肪酸 (PLFA) 标记来表征源自火山灰 (Andisols) 的土壤中的甲烷氧化细菌。三种 Andisols 在 2 ppmv (13) CH4 下孵育长达 18 周，从而能够选择性地表征和定量高亲和力甲烷氧化菌。所有土壤的 PLFA 分布大致相似，但通过 (13) C-PLFA 稳定同位素探测确定的高亲和力甲烷氧化菌群的数量显示出相当大的差异。 (13) C 的大量掺入表明两种土壤中存在非常大的高亲和力甲烷氧化菌群。如此高的值远远超过在类似条件下培养的一系列矿质土壤中观察到的值（10 倍）（Bull 等人，2000 年；Maxfield 等人，2006 年；2008a，b）。研究的三种 Andisols 中的两种也表现出较高但可变的 CH4 氧化速率，范围为 0.03 至 1.58 nmol CH4 g(-1) d.wt。 h(-1) 。这些发现表明，安迪土（Andisols）是一种先前未经研究的高亲和力甲烷氧化细菌的土壤类别，尽管其在全球范围内的覆盖面积较低，但可能会氧化大量的大气甲烷。
Methanotrophic bacteria in soils derived from volcanic ash (Andisols) were characterized via time series (13) C-phospholipid fatty acid (PLFA) labelling. Three Andisols were incubated under 2 ppmv (13) CH4 for up to 18 weeks, thus enabling high-affinity methanotrophs to be selectively characterized and quantified. PLFA profiles from all soils were broadly similar, but the magnitude of the high-affinity methanotrophic populations determined through (13) C-PLFA-stable isotope probing displayed sizeable differences. Substantial incorporation of (13) C indicated very large high-affinity methanotrophic populations in two of the soils. Such high values are far in excess (10×) of those observed for a range of mineral soils incubated under similar conditions (Bull et al., 2000; Maxfield et al., 2006; 2008a, b). Two of the three Andisols studied also displayed high but variable CH4 oxidation rates ranging from 0.03 to 1.58 nmol CH4 g(-1) d.wt. h(-1) . These findings suggest that Andisols, a previously unstudied soil class with respect to high-affinity methanotrophic bacteria, may oxidize significant amounts of atmospheric methane despite their low areal coverage globally.