NEURAL DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS BY INDIRECT CO-CULTURE WITH SCHWANN CELLS
NEURAL DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS BY INDIRECT CO-CULTURE WITH SCHWANN CELLS
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DOI：
10.2298/abs0904703l
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发表时间：
2009-01-01
影响因子：
0.8
通讯作者：
Tan, Zhen
中科院分区：
文献类型：
--
作者：
Li, Xiaojie;Liao, Dapeng;Tan, Zhen
为了研究是否脂肪干细胞（ADSCs）可以只受雪旺细胞（SC）因子诱导的神经分化，我们在transwell培养皿中共培养ADSCs和SC。免疫组化、Western blot和RT-PCR检测共培养1、3、7、14 d的ADSCs中S-100、Nestin和GFAP的基因和蛋白表达。此外，qRT-PCR揭示了上述三种基因表达的相对定量差异。我们认为ADSCs与SC共培养后可以进行神经诱导分化，共培养后开始1 d开始分化，7 d后分化明显，此后一直稳定到第14 d。
To investigate whether adipose-derived stem cells (ADSCs) could be subject to neural differentiation induced only by Schwann cell (SC) factors, we co-cultured ADSCs and SCs in transwell culture dishes. Immunoassaying, Western blot analysis, and RT-PCR were performed (1, 3, 7, 14 d) and the co-cultured ADSCs showed gene and protein expression of S-100, Nestin, and GFAP. Further, qRT-PCR disclosed relative quantitative differences in the above three gene expressions. We think ADSCs can undergo induced neural differentiation by being co-cultured with SCs, and such differentiations begin 1 day after co-culture, become apparent after 7 days, and thereafter remain stable till the 14(th) day.