Occurrence, characteristics, and genetic diversity of azotobacter chroococcum in various soils of southern Poland
Occurrence, characteristics, and genetic diversity of azotobacter chroococcum in various soils of southern Poland
复制标题
波兰南部各种土壤中色球固氮菌的发生、特征和遗传多样性
DOI：
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发表时间：
2012
影响因子：
1.8
通讯作者：
A. Lenart
中科院分区：
文献类型：
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作者：
A. Lenart
固氮菌的存在。土壤中的细菌对植物有有益的影响，但这些细菌的丰度与许多因素有关，特别是土壤的pH值和肥力。本研究对固氮菌的丰度进行了评价。在波兰南部的各种土壤中发现了细菌，并证实了土壤性质与这些细菌的存在之间的关系。诊断试验表明，所有菌株均为嗜铬假单胞菌属。所研究的细菌存在于43%的土壤样本中。细菌内转录间隔区的限制性内切酶分析表明，所选的菌株是相同的。两种指纹分析方法--聚合酶链式反应熔融图谱和随机多态DNA分析方法均显示出较高的群体多样性。定义了4个(聚合酶链式反应MP)和5个(随机扩增多态性DNA)同源簇。菌株分离的来源和它们在集群中的位置之间没有相关性。PCR、MP和RAPD技术可用于嗜铬假单胞菌的种内分化。
The presence of Azotobacter sp. in soils has beneficial effects on plants, but the abundance of these bacteria is related to many factors, especially soil pH and fertility. The presented study evaluated the abundance of Azotobacter sp. in various soils of southern Poland and confirmed the relationship between soil properties and the presence of these bacteria. Diagnostic tests indicate that all isolates belonged to A. chroococcum species. The studied bacteria were present in 43% of soil samples. Restriction analysis of the bacterial Internal Transcribed Spacer region indicate that the selected isolates were identical. Two fingerprinting methods, PCR Melting Profile and Random Analysis of Polymorphic DNA, revealed high population diversity. Four (PCR MP) and five (RAPD) congruent clusters were defined. No correlation was found between the sources of strain isolation and their positions in clusters. PCR MP and RAPD techniques appeared to be useful in intraspecies differentiation of A. chroococcum.