Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses
Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses
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DOI：
10.1182/blood.v97.11.3498
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发表时间：
2001-06-01
期刊：
影响因子：
20.3
通讯作者：
Kikutani, H
中科院分区：
文献类型：
--
作者：
Wang, XS;Kumanogoh, A;Kikutani, H
CD 100/Sema 4D是一个150 kd的跨膜蛋白，属于semaphorin家族。CD 100与CD 72的相互作用对免疫系统至关重要。在CD 100缺陷小鼠中，针对T细胞依赖性抗原的特异性抗体的产生严重受损，但针对T细胞非依赖性抗原的特异性抗体的产生不受影响。在这里，一个功能性的可溶性CD 100蛋白（sCD 100）从激活的淋巴细胞释放的报告。从活化的T淋巴细胞培养上清中检测到sCD 100。无论是从活化的T淋巴细胞培养上清中亲和纯化的，还是由驼鹿CD 100的胞外区与人IgG 1 Fc融合而成的重组sCD 100蛋白（CD 100-Fc），sCD 100均显著增强CD 40诱导的B细胞应答。免疫组小鼠血清中sCD 100水平与自身抗体滴度呈显著正相关，免疫组小鼠血清中sCD 100水平与自身抗体滴度呈显著负相关。这项研究的结果表明，sCD 100在免疫反应中的潜在作用，包括抗体的产生和自身免疫性疾病。（Blood，2001;91：3498-3504）（C）2001年美国血液学会。
CD100/Sema4D is a 150-kd transmembrane protein that belongs to the semaphorin family. The interaction of CD100 with CD72 is critical for the immune system. in CD100-deficient mice, the production of specific antibodies against T-cell-dependent antigens is severely impaired, but not against T-cell-independent antigens. Here, a functional soluble CD100 protein (sCD100) released from activated lymphocytes is reported. sCD100 was detected in culture supernatants of activated lymphocytes, Either affinity-purified from supernatants of activated T-cells, or produced as a recombinant sCD100 protein consisting of the extracellular region of the moose CD100 fused to the human IgG1 Fc(CD100-Fc), sCD100 significantly enhanced CD40-induced B-cell responses. Furthermore, sCD100 was detected either in sera of mice immunized with T-cell-dependent antigens, or in sera of MRL/lpr mice, but not in sera of mice immunized with T-cell-independent anti-gens, A significant correlation was observed between the level of sCD100 and the titer of autoantibodies in the serum of MRL/lpr mice. This study's findings suggest a potential role for sCD100 in immune responses, including production of antibody, and autoimmune diseases. (Blood, 2001;91: 3498-3504) (C) 2001 by The American Society of Hematology.