Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma
Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma
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DOI：
10.1155/2020/3574261
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发表时间：
2020-09-09
影响因子：
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通讯作者：
Wu, Qing
中科院分区：
文献类型：
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作者：
Cao, Ting;Yi, Shi-jie;Wu, Qing
背景微脂质体维持（MCM）复合物，MCM 2 -7，被揭示参与多种细胞过程，并在人类癌症的发展和进展中起关键作用。然而，MCM复杂的肝癌（HCC）仍然不清楚阐述。在本研究中，我们通过生物信息学的方法，从mRNA和蛋白质水平进行了研究。我们还探讨了MCM 2 -7的预后价值、遗传改变、相互作用网络和功能富集。Western blot检测肝癌细胞系中MCM的表达，并分析MCM之间的相关性。与正常肝组织相比，HCC组织中的MCM 2 -7显著增加。MCM 2 -7高表达与预后不良呈正相关。然而，MCM 2 -7的改变与较差的OS不相关。与正常肝细胞系相比，HCC细胞系中的MCMs均增加。此外，肝癌细胞系中MCM之间存在正相关性。结论。MCM复合物在肝癌组织和细胞系中表达增加，并与预后呈负相关，可能是肝癌的重要生物标志物。
Background. The microliposome maintenance (MCM) complex, MCM2-7, is revealed to be involved in multiple cellular processes and plays a key role in the development and progression of human cancers. However, the MCM complex remains poorly elaborated in hepatic carcinoma (HCC).Methods. In the study, we found the mRNA and protein level by bioinformatics. We also explored the prognostic value, genetic alteration, interaction network, and functional enrichment of MCM2-7. The MCM expression and correlation among these MCMs in HCC cell lines were identified by western blot.Results. MCM2-7 was significantly increased in HCC tissues compared to normal liver tissues. The high level of MCM2-7 had a positive correlation with poor prognosis. However, MCM2-7 alterations were not correlated with poor OS. MCMs were both increased in HCC cell lines compared to the normal hepatocyte cell line. Furthermore, the positive correlation was found among MCMs in HCC cell lines.Conclusions. The MCM complex was increased in HCC tissues and cell lines and negatively correlated with prognosis, which might be important biomarkers for HCC.