Stomatal density affects rice mesophyll cell size and shape and modulates a conserved pattern of cells through the leaf
Stomatal density affects rice mesophyll cell size and shape and modulates a conserved pattern of cells through the leaf
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DOI：
10.1101/2022.11.09.515764
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发表时间：
2022-11
期刊：
影响因子：
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通讯作者：
Sloan Jen;Im-Chai Saranrat;Qi Yang Ngai;Xiao Yi;Armand Jodie;Matthew J. Wilson;Xin-Guang Zhu;A. Fleming
中科院分区：
文献类型：
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作者：
Sloan Jen;Im-Chai Saranrat;Qi Yang Ngai;Xiao Yi;Armand Jodie;Matthew J. Wilson;Xin-Guang Zhu;A. Fleming
叶肉的结构影响光、二氧化碳和水在叶片中的传播方式，从而影响光合作用和蒸腾作用的速率。最近在小麦和拟南芥上的研究表明，气孔密度和气孔活动影响叶肉细胞的结构，这与气孔气体流动可以调节内部细胞的生长和分离以协调叶片结构和功能的假说一致。为了研究这种情况是否也发生在大米中，大米是世界上很大一部分人口的主食作物，我们检查了气孔密度改变的水稻突变体的叶肉结构。我们的数据表明，气孔功能调节水稻的叶肉结构。叶肉叶裂程度的变化是造成叶肉结构改变的主要原因，这表明通过气孔的叶片气体通量的改变影响了与水稻气体交换能力直接相关的细胞形状的一个方面。此外，我们的分析揭示了水稻叶肉各层的细胞大小、形状和轴性的一种以前未报道的潜在模式，进一步的研究表明，这种模式存在于一系列水稻物种和品种中。讨论了这种叶肉纹样的潜在来源和意义。亮点我们描述了一种以前没有报道过的水稻叶片细胞模式，并表明它是由气孔调节的。这些结果为了解叶片的结构和功能提供了新的线索。
The structure of the mesophyll influences how light, CO2 and water travels inside a leaf, affecting the rates of both photosynthesis and transpiration. Recent studies in wheat and Arabidopsis have shown that the structure of the mesophyll is influenced by the density and activity of stomata, consistent with the hypothesis that gas flow via stomata can modulate internal cell growth and separation to co-ordinate leaf structure and function. To investigate whether this also occurs in rice, a staple food crop for a large fraction of the world’s population, we examined mesophyll structure in rice mutants with altered stomatal density. Our data show that stomatal function modulates mesophyll structure in rice. Variation in the degree of mesophyll lobing made a major contribution to altered mesophyll structure, suggesting that modified leaf gas flux through stomata influences an aspect of cell shape directly linked to gas exchange capacity in rice. In addition, our analysis revealed a previously unreported underlying pattern in cell size, shape and axiality across layers of the rice mesophyll, which further investigation revealed is present in a range of rice species and cultivars. The potential origin and significance of this mesophyll patterning are discussed. HIGHLIGHT We describe a previously unreported cellular pattern in rice leaves and show that it is modulated by stomata. These results shed new light on leaf structure and function.