Acclimation Improves Methane Production from Molasses Wastewater with High Salinity in an Upflow Anaerobic Filter Reactor: Performance and Microbial Community Dynamics
Acclimation Improves Methane Production from Molasses Wastewater with High Salinity in an Upflow Anaerobic Filter Reactor: Performance and Microbial Community Dynamics
复制标题
驯化提高了上流式厌氧过滤反应器中高盐度糖蜜废水的甲烷产量：性能和微生物群落动态
DOI：
10.1007/s12010-020-03236-7
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发表时间：
2020-02-04
影响因子：
3
通讯作者：
Kida, Kenji
中科院分区：
文献类型：
--
作者：
Chen, Ya-Ting;Yu, Na;Kida, Kenji
研究了上流式厌氧滤池（UAF）反应器在糖蜜高盐废水高温甲烷发酵中的性能。未稀释废水的高盐度（~ 45 mS/cm）完全抑制了沼气的产生。采用糖蜜废水梯度稀释的驯化策略，逐步增加盐胁迫。因此，沼气生产得到恢复，仅受到未稀释废水的高盐度的轻微抑制。该反应器稳定地实现了5 g/L/天的高总有机碳（TOC）负荷率，TOC去除效率约为60%。驯化逐渐增加的盐胁迫导致一些耐盐微生物的相对丰度，如Arcobacter，Tissierella和Ruminococcaceae的细菌，随着它们的水解和产酸能力适应盐度的增加而增加。此外，氢营养型产甲烷菌，特别是甲烷囊菌，比乙酸型产甲烷菌表现出更强的耐盐性。这些结果表明，驯化的发酵微生物群落的高盐度是一个有效的策略，以提高甲烷生产的高盐糖蜜废水在高温UAF反应器。
This study evaluated the performance of an upflow anaerobic filter (UAF) reactor in the thermophilic methane fermentation of hypersaline molasses wastewater. The high salinity (~ 45 mS/cm) of the undiluted wastewater completely inhibited the biogas production. An acclimation strategy involving gradient dilution of the molasses wastewater was implemented to gradually increase the salt stress. Consequently, the biogas production was recovered, inhibited only slightly by the high salinity of the undiluted wastewater. The reactor steadily achieved a high total organic carbon (TOC) loading rate of 5 g/L/day, with approximately 60% TOC removal efficiency. Acclimation to the gradually increased salt stress leads to a relative abundance of some halotolerant microbes, such as bacteria fromArcobacter,Tissierella, andRuminococcaceae, which increased as their hydrolytic and acidogenic abilities adjusted to the incremental increase in salinity. Additionally, hydrogenotrophic methanogens, especiallyMethanoculleus, showed greater resistance to hypersalinity than aceticlastic methanogens. These results suggest that acclimation of the fermentation microbial community to hypersalinity is an effective strategy to improve methane production from hypersaline molasses wastewater in thermophilic UAF reactors.