INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance
INDETERMINATE SPIKELET1 Recruits Histone Deacetylase and a Transcriptional Repression Complex to Regulate Rice Salt Tolerance
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不确定的 SPIKELET1 招募组蛋白脱乙酰酶和转录抑制复合物来调节米盐耐受性
DOI：
10.1104/pp.18.00324
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发表时间：
2018-10-01
期刊：
影响因子：
7.4
通讯作者：
Chen, Tao
中科院分区：
文献类型：
--
作者：
Cheng, Xiliu;Zhang, Shaoxuan;Chen, Tao
盐胁迫信号的感知和传导对于植物的存活、生长和繁殖至关重要。因此，鉴定盐胁迫信号通路的组分对水稻抗盐分子育种具有重要意义。在这里，我们报告了一个apetala 2/乙烯反应因子转录因子INTRAATE SPIKELET 1（IDS 1）的鉴定及其在水稻耐盐性调控中的作用。通过遗传筛选和表型分析，我们证明了IDS 1具有转录抑制活性，并在水稻耐盐性中起负调节作用。为了确定潜在的下游目标基因的IDS 1调控，我们进行了染色质免疫沉淀（ChIP）测序和ChIP-定量PCR分析，发现IDS 1可能直接与非生物胁迫响应基因，包括LEA 1（晚期胚胎发生丰富蛋白质1）和SOS 1（盐过度敏感1），这是调控水稻耐盐性的关键基因启动子区域中的GCC盒含基序。IDS 1与转录辅阻遏物topless-related 1和组蛋白去乙酰化酶HDA 1相互作用，抑制LEA 1和SOS 1的表达。对LEA 1和SOS 1启动子上组蛋白H3乙酰化状态和RNA聚合酶II占据的分析进一步确定了IDS 1转录抑制活性的分子基础。我们的研究结果阐明了表观遗传机制，IDS 1调节盐胁迫信号，以及作为盐耐水稻。
Perception and transduction of salt stress signals are critical for plant survival, growth, and propagation. Thus, identification of components of the salt stress-signaling pathway is important for rice (Oryza sativa) molecular breeding of salt stress resistance. Here, we report the identification of an apetala2/ethylene response factor transcription factor INDETERMINATE SPIKELET1 (IDS1) and its roles in the regulation of rice salt tolerance. By genetic screening and phenotype analysis, we demonstrated that IDS1 conferred transcriptional repression activity and acted as a negative regulator of salt tolerance in rice. To identify potential downstream target genes regulated by IDS1, we conducted chromatin immunoprecipitation (ChIP) sequencing and ChIP-quantitative PCR assays and found that IDS1 may directly associate with the GCC-box-containing motifs in the promoter regions of abiotic stress-responsive genes, including LEA1 (LATE EMBRYOGENESIS ABUNDANT PROTEIN1) and SOS1 (SALT OVERLY SENSITIVE1), which are key genes regulating rice salt tolerance. IDS1 physically interacted with the transcriptional corepressor topless-related 1 and the histone deacetylase HDA1, contributing to the repression of LEA1 and SOS1 expression. Analyses of histone H3 acetylation status and RNA polymerase II occupation on the promoters of LEA1 and SOS1 further defined the molecular foundation of the transcriptional repression activity of IDS1. Our findings illustrate an epigenetic mechanism by which IDS1 modulates salt stress signaling as well as salt tolerance in rice.