Lidocaine reversibly inhibits fertilization in Chlamydomonas: a possible role for calcium in sexual signalling.
Lidocaine reversibly inhibits fertilization in Chlamydomonas: a possible role for calcium in sexual signalling.
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利多卡因可逆地抑制衣藻的受精：钙在性信号中的可能作用。
DOI：
10.1083/jcb.94.3.607
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Clausell,A
中科院分区：
文献类型：
--
作者：
Snell,WJ;Buchanan,M;Clausell,A
鞭毛粘附诱导的信号在双鞭毛藻的交配反应中发出，莱茵衣藻，启动细胞壁降解酶的释放，交配结构的激活和细胞融合。这个信号的性质是未知的，但它可能是介导的粘附诱导的变化（激活）的鞭毛尖端。本文报道的研究表明，利多卡因是一种局部麻醉剂，据报道可干扰二价阳离子穿过细胞膜的运动，可逆地阻断细胞壁丢失和配子融合，而不阻断粘附或鞭毛尖端激活。在这些实验中，利多卡因抑制初始速率和壁损失和合子形成的程度。对麻痹鞭毛突变体pf 17的配子的研究表明，在抑制剂浓度下，利多卡因也能阻断鞭毛表面运动（表现为聚苯乙烯珠的运动），这也阻止了配子融合。阻断细胞融合所需的利多卡因浓度取决于培养基中钙或镁的浓度。在不添加钙的情况下，0.5 mM利多卡因抑制融合70%。在0.5 mM钙中，0.5 mM利多卡因对融合没有影响，90%抑制需要2 mM利多卡因。结果表明，二价阳离子可能在衣原体的性信号中发挥关键作用。
A flagellar adhesion-induced signal sent during the mating reaction of the biflagellate alga, Chlamydomonas reinhardtii, initiates release of cell-wall-degrading enzymes, activation of mating structures, and cell fusion. The nature of this signal is unknown, but it may be mediated by an adhesion-induced change (activation) of flagellar tips. The studies reported here show that lidocaine, a local anesthetic that is reported to interfere with the movement of divalent cations across cell membranes, reversibly blocks cell wall loss and gametic fusion without blocking adhesion or flagellar tip activation. In these experiments lidocaine inhibited both the initial rates and the extent of wall loss and zygote formation. Studies with gametes of a paralyzed flagellar mutant, pf 17, revealed that lidocaine also blocked flagellar surface motility (visualized as movement of polystyrene beads) at concentrations of the inhibitor which also prevented gametic fusion. The concentration of lidocaine required to block cell fusion was dependent on the concentration of calcium or magnesium in the medium. In the absence of added calcium, 0.5 mM lidocaine inhibited fusion by 70%. In 0.5 mM calcium, 0.5 mM lidocaine had no effect on fusion and 2 mM lidocaine was required for 90% inhibition. The results suggest that divalent cations may play a critical role in sexual signalling in Chlamydomonas.