Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library.
Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library.
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通过筛选 CRISPR/Cas9 文库获得的 rhEGF 生物测定反应细胞。
DOI：
10.1038/s41598-019-40381-4
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发表时间：
2019
影响因子：
4.6
通讯作者：
Wang Junzhi
中科院分区：
文献类型：
--
作者：
Qin Xi;Yao Wenrong;Shi Xinchang;Liu Lan;Huang Fang;Ding Youxue;Zhou Yong;Yu Lei;Jia Chuncui;Li Shanhu;Rao Chunming;Wang Junzhi
重组蛋白产品的生物学检测是保证蛋白质有效性的重要检测手段。一些重组蛋白产品在其生物测定中没有使用细胞，而是使用动物模型，而另一些则没有合适的方法。在这里，我们开发了一种方法来获得用于蛋白质生物测定的响应细胞。在筛选CRISPR/Cas9文库后，我们获得了在rhEGF（重组人表皮生长因子）存在下比对照细胞生长更快的响应细胞系。我们用此细胞系进行rhEGF的生物测定。与对照细胞相比，该细胞系的操作时间缩短了2天，并且具有较低的干扰。反应细胞系更适合于rhEGF的生物测定。
Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF.