Preferential release of proinsulin relative to insulin in non-insulin-dependent diabetes mellitus.
Preferential release of proinsulin relative to insulin in non-insulin-dependent diabetes mellitus.
复制标题
在非胰岛素依赖型糖尿病中，胰岛素原相对于胰岛素优先释放。
DOI：
10.1530/acta.0.1190549
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
W. Creutzfeldt
中科院分区：
文献类型：
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作者：
C. Deacon;S. Schleser;M. Ballmann;B. Willms;J. Conlon;W. Creutzfeldt
一种使用人类胰岛素原特异性抗血清的放射免疫测定法，已被用于研究非肥胖非胰岛素依赖型糖尿病（NIDDM）患者血清胰岛素原对低（25 g）和高（75 g）口服葡萄糖负荷的反应。糖尿病患者仅接受饮食治疗（N = 8）或接受口服降糖药治疗（N = 8），研究期间不中断治疗。在禁食状态下，药物治疗组胰岛素原浓度（31 +/- 3 pmol/l (SEM)）高于年龄和体重匹配的健康受试者（22 +/- 2 pmol/l, N = 10），但饮食治疗组胰岛素原浓度（25 +/- 3 pmol/l）无显著差异（P < 0.05）。在25 g和75 g葡萄糖负荷下，两组糖尿病患者血清免疫反应性胰岛素和c肽浓度的升高相对于对照组受到损害和延迟。然而，在180分钟内的任何时间点，NIDDM患者的血清胰岛素原反应与对照组相比没有显著差异，除了接受25 g葡萄糖治疗的患者在摄入后150分钟和180分钟时胰岛素原浓度升高（P < 0.05）。由此得出结论，与健康受试者相比，NIDDM与葡萄糖反应中胰岛素原的释放不相关，但胰岛保持释放胰岛素原的能力优于释放胰岛素的能力。
A radioimmunoassay, using an antiserum that is specific for human proinsulin, has been used to study the response of serum proinsulin to low (25 g) and high (75 g) oral glucose loads in non-obese patients with non-insulin-dependent diabetes mellitus (NIDDM). Diabetic patients were treated by diet only (N = 8) or were receiving oral anti-hyperglycemic agents (N = 8) and therapy was not interrupted during the study. In the fasted state, proinsulin concentrations were higher (P less than 0.05) in the drug-treated patients (31 +/- 3 pmol/l (SEM)) compared with age- and weight-matched healthy subjects (22 +/- 2 pmol/l; N = 10), but concentrations in the diet-treated patients 25 +/- 3 pmol/l) were not significantly different. Following 25 g and 75 g glucose loads, the rises in serum immunoreactive insulin and C-peptide concentrations in both groups of diabetic patients were impaired and delayed relative to those in the control subjects. The responses of serum proinsulin, however, were not significantly different in the NIDDM patients compared with controls at any time point up to 180 min except in the case of drug-treated patients receiving 25 g of glucose who had elevated (P less than 0.05) proinsulin concentrations at 150 min and 180 min after ingestion. It is concluded that NIDDM is not associated with an exaggerated release of proinsulin in response to glucose compared with healthy subjects, but the islets have maintained the ability to release proinsulin better than the ability to release insulin.