Comparison of near-infrared fluorescent deoxyglucose probes with different dyes for tumor diagnosis in vivo
Comparison of near-infrared fluorescent deoxyglucose probes with different dyes for tumor diagnosis in vivo
复制标题
DOI：
10.1002/cmmi.496
复制
发表时间：
2012-05-01
影响因子：
--
通讯作者：
Gu, Yueqing
中科院分区：
文献类型：
--
作者：
Guo, Jing;Du, Changli;Gu, Yueqing
葡萄糖在细胞能量代谢中起着核心作用。恶性肿瘤的糖酵解速率高于正常组织。本研究将两种水溶性不同的近红外荧光染料Cypate和ICG-Der-02分别与2-氨基-2-脱氧-d-葡萄糖（2DG）偶联，形成Cypate-2DG和ICG-Der-02-2DG，用于裸鼠肿瘤的近红外荧光成像。比较了两种近红外荧光2DG探针的清晰路线和肿瘤靶向能力。结果显示，具有较高亲水性的ICG-Der-02-2DG比亲脂性更高的Cypate-2DG更快地被肾脏清除。Cypate-2DG与ICG-Der-02-2DG相比，具有更慢但更强的肿瘤靶向能力。为了研究探针的靶向能力与癌细胞的葡萄糖转运蛋白（GLUT 1）表达水平之间的相关性，在表达不同水平GLUT 1的MCF-7/雌二醇、U87 MG、MCF-7和MDA-MB-435肿瘤异种移植物中评估Cypate-2DG在肿瘤中的积累。结果表明Cypate-2DG和ICG-Der-02-2DG对所有检查的肿瘤都具有肿瘤靶向能力，并且与GLUT 1表达成比例相关。研究结果表明，这些分子探针对肿瘤和葡萄糖相关病变的光学成像具有广泛的适用性。版权所有（c）2012约翰威利父子有限公司
Glucose plays a central role in the cellular energy metabolism. Malignant tumors exhibit an elevated rate of glycolysis over normal tissues. In this study, two near-infrared fluorescent dyes, Cypate and ICG-Der-02, with different water solubility, were conjugated to 2-amino-2-deoxy-d-glucose (2DG) to form Cypate-2DG and ICG-Der-02-2DG, respectively, for NIR fluorescent imaging of tumors in nude mice. The clear routes and tumor targeting abilities of the two NIR fluorescent 2DG probes were compared. Results showed that ICG-Der-02-2DG with higher hydrophilicity was cleared faster by kidneys than the more lipophilic Cypate-2DG. Cypate-2DG had slower but stronger tumor targeting ability compared with ICG-Der-02-2DG. To investigate the correlation between the targeting ability of the probe and the glucose transporter (GLUT1) expression levels of cancer cells, the accumulation of Cypate-2DG in tumors was assessed in MCF-7/estradiol, U87MG, MCF-7 and MDA-MB-435 tumor xenografts, which express different levels of GLUT1. The results show that both Cypate-2DG and ICG-Der-02-2DG possess tumor targeting ability on all the tumors examined, with a proportional correlation to GLUT1 expression. The findings demonstrate the broad applicability of these molecular probes for optical imaging of tumors and glucose-related pathologies. Copyright (c) 2012 John Wiley & Sons, Ltd.