Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production
Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production
复制标题
中断工业酒精酵母中的甘油途径以提高乙醇产量
DOI：
10.1007/s00253-008-1777-7
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发表时间：
2009-02-01
影响因子：
5
通讯作者：
Shi, Gui-Yang
中科院分区：
文献类型：
--
作者：
Guo, Zhong-peng;Zhang, Liang;Shi, Gui-Yang
工业酵母Saccharomycescerevisiae CICIMY 0086中编码NAD（+）依赖性甘油-3-磷酸脱氢酶同工酶的两个同源基因GPD 1和GPD 2已被删除。在酒精发酵条件下对获得的两种突变株gpd 1 Delta和gpd 2 Delta进行了研究。gpd 1 Delta突变体的表达率为4.29%，（相对于消耗的底物的量）甘油产量和6.83%的降低（相对于消耗的底物的量）增加了乙醇产率，而gpd 2 Delta突变体表现出7.95%的乙醇产率。（相对于消耗的底物的量）甘油产量降低7.41%，（相对于消耗的底物的量）与亲本菌株相比增加了乙醇产率。在指数生长期，两个突变体的生长速率略低于野生型，而ANG 1（gpd 1 Delta）和甘油产量的减少不伴随着菌株ANG 1（gpd 1 Delta）的蛋白质含量的任何下降，但在菌株ANG 2（gpd 2 Delta）中略有下降。同时，与亲本菌株相比，在菌株ANG 1（gpd 1 Delta）和ANG 2（gpd 2 Delta）中观察到乙酸形成的显著减少。因此，有可能通过阻断甘油途径来提高工业酒精酵母的乙醇产量。
The two homologous genes GPD1 and GPD2, encoding two isoenzymes of NAD(+)-dependent glycerol-3-phosphate dehydrogenase in industrial yeast Saccharomyces cerevisiae CICIMY0086, had been deleted. The obtained two kinds of mutants gpd1 Delta and gpd2 Delta were studied under alcoholic fermentation conditions. gpd1 Delta mutants exhibited a 4.29% (relative to the amount of substrate consumed) decrease in glycerol production and 6.83% (relative to the amount of substrate consumed) increased ethanol yield while gpd2 Delta mutants exhibited a 7.95% (relative to the amount of substrate consumed) decrease in glycerol production and 7.41% (relative to the amount of substrate consumed) increased ethanol yield compared with the parental strain. The growth rate of the two mutants were slightly lower than that of the wild type under the exponential phase whereas ANG1 (gpd1 Delta) and the decrease in glycerol production was not accompanied by any decline in the protein content of the strain ANG1 (gpd1 Delta) but a slight decrease in the strain ANG2 (gpd2 Delta). Meanwhile, dramatic decrease of acetate acid formation was observed in strain ANG1 (gpd1 Delta) and ANG2 (gpd2 Delta) compared to the parental strain. Therefore, it is possible to improve the ethanol yield by interruption of glycerol pathway in industrial alcoholic yeast.