Unipept 4.0: Functional Analysis of Metaproteome Data
Unipept 4.0: Functional Analysis of Metaproteome Data
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DOI：
10.1021/acs.jproteome.8b00716
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发表时间：
2019-02-01
影响因子：
4.4
通讯作者：
Mesuere, Bart
中科院分区：
文献类型：
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作者：
Gurdeep Singh, Robbert;Tanca, Alessandro;Mesuere, Bart
Unipept(https://unipept.ugent.be))是一个用于代谢蛋白质组数据分析的Web应用程序，最初的重点是MS/MS样品的基于胰酶多肽的生物多样性分析。由于代谢蛋白质组学的真正潜力在于洞察复杂环境样本的表达功能，Unipept的4.0版本引入了基于GO项和EC编号的互补功能分析。将这一新的功能分析与现有的生物多样性分析相结合是延长管道的一项重要资产。作为概念的证明，用Unipept 4.0重新分析了来自15名健康受试者的人类粪便MetaProtem数据集，得到了快速、详细和直接的分类单元特异性催化功能的表征，这与先前基于BLAST的相同数据的功能分析的结果一致。
Unipept (https://unipept.ugent.be) is a web application for metaproteome data analysis, with an initial focus on tryptic-peptide-based biodiversity analysis of MS/MS samples. Because the true potential of metaproteomics lies in gaining insight into the expressed functions of complex environmental samples, the 4.0 release of Unipept introduces complementary functional analysis based on GO terms and EC numbers. Integration of this new functional analysis with the existing biodiversity analysis is an important asset of the extended pipeline. As a proof of concept, a human faecal metaproteome data set from 15 healthy subjects was reanalyzed with Unipept 4.0, yielding fast, detailed, and straightforward characterization of taxon-specific catalytic functions that is shown to be consistent with previous results from a BLAST-based functional analysis of the same data.