PLANT GLUTAMINE-SYNTHETASE COMPLEMENTS A GLNA MUTATION IN ESCHERICHIA-COLI
PLANT GLUTAMINE-SYNTHETASE COMPLEMENTS A GLNA MUTATION IN ESCHERICHIA-COLI
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DOI：
10.1126/science.2871626
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发表时间：
1986-06-06
期刊：
影响因子：
56.9
通讯作者：
GOODMAN, HM
中科院分区：
文献类型：
--
作者：
DASSARMA, S;TISCHER, E;GOODMAN, HM
苜蓿谷氨酰胺合成酶基因在大肠杆菌中得到了表达。通过对谷氨酰胺合成酶缺陷突变体的功能性遗传互补和免疫印迹分析，证明了苜蓿谷氨酰胺合成酶在大肠杆菌中的合成酶。这些结果将有助于蛋白质工程和结构功能分析的植物酶。
A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translocation signals to a complete alfalfa glutamine synthetase coding sequence. Synthetase of the alfalfa glutamine synthetase enyzme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.