Innate expression of antimicrobial peptides does not explain genotypic diversity in resistance to fungal brood parasites in the honey bee
Innate expression of antimicrobial peptides does not explain genotypic diversity in resistance to fungal brood parasites in the honey bee
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DOI：
10.1007/s13592-015-0388-4
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发表时间：
2016-03-01
期刊：
影响因子：
2.4
通讯作者：
Hughes, William O. H.
中科院分区：
文献类型：
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作者：
Evison, Sophie E. F.;Fazio, Geraldine;Hughes, William O. H.
宿主对寄生虫抗性的遗传基础是宿主-寄生虫共同进化的一个基本方面，但其确切机制往往仍不清楚。在这里，我们遵循从以前的研究遗传介导的变化，在两个常见的真菌寄生虫，导致蜜蜂的白垩和stonebrood。我们评估了是否可以解释的基线免疫能力不同的幼虫基因型相关的两个关键的免疫基因的组成型表达与观察到的每个幼虫基因型的抗性水平，以四种不同的真菌幼虫寄生虫的遗传介导的变化。我们发现幼虫基因型之间的显着变化，但不防御素2的组成型表达，但尽管在老年幼虫的基因表达和抗性水平之间的负相关性的建议，有没有一致的证据表明，基线abaecin的表达是一个相关的预测这些寄生虫的抗性。这些结果表明，组成型表达的abaecin似乎有一个遗传基础，在蜜蜂幼虫，但机制比先天表达的抗菌肽可能是更重要的防御特定的真菌幼虫寄生虫在这里评估。
The genetic basis of host resistance to parasites is a fundamental aspect of host-parasite co-evolution, yet the precise mechanisms often remain unclear. Here, we follow on from a previous study on the genetically mediated variation in resistance to two common fungal brood parasites that cause chalkbrood and stonebrood in the honey bee. We assessed whether genetically mediated variation in resistance can be explained by the baseline immunocompetence of different larval genotypes by correlating the constitutive expression of two key immune genes with the observed level of resistance of each larval genotype to four different fungal brood parasites. We found significant variation between larval genotypes in the constitutive expression of abaecin but not defensin 2, but despite a suggestion of negative correlations between gene expression and resistance level in older larvae, there was no consistent evidence that baseline abaecin expression is a relevant predictor of resistance to these parasites. These results suggest that the constitutive expression of abaecin appears to have a genetic basis in honey bee larvae but that mechanisms other than innate expression of antimicrobial peptides might be more important in defence against the specific fungal brood parasites assessed here.