Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
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DOI：
10.1038/nmeth1146
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发表时间：
2008-01-01
期刊：
影响因子：
48
通讯作者：
Perrimon, Norbert
中科院分区：
文献类型：
--
作者：
Ni, Jian-Quan;Markstein, Michele;Perrimon, Norbert
近年来，果蝇中发夹结构的条件表达已成为功能基因组研究的一种选择方法。迄今为止，上游激活位点驱动的 RNA 干扰构建体已使用 P 元件介导的转化随机插入基因组中，这可能因表达可变而导致假阴性。为了避免这个问题，我们开发了基于phiC31位点特异性整合方法的转基因RNA干扰载体。
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.