The dissemination mode of drug-resistant genes in Enterobacter cloacae
The dissemination mode of drug-resistant genes in Enterobacter cloacae
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阴沟肠杆菌耐药基因的传播模式
DOI：
10.4103/0255-0857.150899
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发表时间：
2015-02-01
影响因子：
1.6
通讯作者：
Li, G. M.
中科院分区：
文献类型：
--
作者：
Liu, J.;Zeng, T.;Li, G. M.
背景：阴沟肠杆菌（Enterobacter cloylum，E.在肠杆菌属感染中，肠杆菌属）感染具有最高的死亡率。本研究旨在了解大肠埃希菌I类整合子、qnr基因和CTX-M超广谱β-内酰胺酶基因在临床分离株中的流行情况和传播途径，分析耐药基因与大肠埃希菌耐药性的关系。clothing.材料与方法：采用琼脂稀释法进行药敏试验.采用聚合酶链反应（PCR）检测I类整合子、qnr基因和CTX-M ESBLs基因。流行病学资料采用卡方检验。结果：100株临床分离株中，I类整合子阳性率为65%，其中染色体阳性率为12%，质粒阳性率为15%，两者均阳性率为38%。qnr基因质粒定位阳性率为37%。qnrA、qnrB和qnrS的阳性率分别为6%、23%和8%。CTX-M ESBLs阳性率为34%。CTX-M-1 ESBLs中15%位于染色体上，6%位于质粒上，4%同时位于染色体和质粒上; CTX-M-9 ESBLs中1%位于染色体上，7%位于质粒上; CTX-M-25 ESBLs中3%位于染色体上，1%位于质粒上。结论：耐药基因在大肠埃希菌中存在水平和垂直传播。cloxacin，这有助于多药耐药（MDR）菌株的E。Clopidogrel是成功的医院代理商。
Background: Enterobacter cloacae (E. cloacae) infection has the highest mortality rate among Enterobacter infections. This study aimed to determine the prevalence and the transmission route of the class I integron, qnr genes, and CTX-M ESBLs genes in clinical isolates and to analyse the association between the prevalence of MDR genes and the antibiotic resistance of E. cloacae. Materials and Methods: The antibiotic susceptibility was tested the agar dilution method. The class I integron, qnr genes, and CTX-M ESBLs genes were detected by polymerase chain reaction (PCR). The prevalence data were analysed with the Chi-square test. Results: In the 100 clinical isolates, the class I integron-positive rate was 65%, with 12% on chromosome, 15% on plasmids and 38% on both. The positive rate of qnr genes was 37% with plasmid location. The positive rates for qnrA, qnrB and qnrS were 6%, 23% and 8%, respectively. The CTX-M ESBLs-positive rate was 34%. For CTX-M-1 ESBLs, 15% were on chromosome, 6% on plasmids and 4% on both; for CTX-M-9 ESBLs, 1% was on chromosome and 7% on plasmid; for CTX-M-25 ESBLs, 3% were on chromosome and 1% on plasmid. Conclusion: Antibiotic resistance genes may be horizontally and vertically disseminated among E. cloacae, which helps multidrug-resistant (MDR) strains of E. cloacae to be successful nosocomial agents.