ALZHEIMERS-DISEASE - TAU PROTEINS, THE PROMOTING FACTORS OF MICROTUBULE ASSEMBLY, ARE MAJOR COMPONENTS OF PAIRED HELICAL FILAMENTS
ALZHEIMERS-DISEASE - TAU PROTEINS, THE PROMOTING FACTORS OF MICROTUBULE ASSEMBLY, ARE MAJOR COMPONENTS OF PAIRED HELICAL FILAMENTS
复制标题
DOI：
10.1016/0022-510x(86)90167-x
复制
发表时间：
1986-12-01
影响因子：
4.4
通讯作者：
DEFOSSEZ, A
中科院分区：
文献类型：
--
作者：
DELACOURTE, A;DEFOSSEZ, A
针对成对螺旋丝（PHF）产生兔抗血清。这种抗血清在阿尔茨海默病新皮质和海马切片中，在光学显微镜水平上对神经原纤维缠结 (NFT) 进行特异性染色，在电子显微镜水平上对 PHF 进行特异性染色。我们通过免疫细胞化学、免疫印迹和免疫吸附研究了该抗血清识别的抗原的性质。这些方法表明，抗 PHF 特异性标记一组低分子量 65-50 kDa 微管相关蛋白，称为 Tau 蛋白，是微管组装的促进因子。此外，Tau 蛋白的抗血清也会对 NFT 进行染色。结论是神经原纤维缠结很可能由聚集的 Tau 蛋白组成。这一过程可能是由于 Tau 蛋白合成异常或某些锥体神经元的未知病变导致 Tau 蛋白聚集。
A rabbit antiserum was raised against paired helical filaments (PHF). This antiserum stains specifically neurofibrillary tangles (NFT) at the light-microscopic level and PHF at the electron-microscopic level in sections of Alzheimer neocortex and hippocampus. We studied the nature of the antigens recognized by this antiserum by immunocytochemistry, immunoblots and immunoadsorption. These approache showed that the anti-PHF specifically labels a set of low molecular weight 65-50 kDa microtuble-associated proteins, named Tau proteins, which are promoting factors of microtubule assembly. Furthermore, antisera against Tau proteins stained NFT. It is concluded that neurofibrillary tangles are very likely composed of aggregated Tau proteins. This process might be due to an abnormal Tau protein synthesis or to an unknown lesion of certain pyramidal neurons leading to an aggregation of Tau proteins.