Theory of optical chromatography
Theory of optical chromatography
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DOI：
10.1021/ac970079z
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发表时间：
1997-07-15
影响因子：
7.4
通讯作者：
Imasaka, T
中科院分区：
文献类型：
--
作者：
Kaneta, T;Ishidzu, Y;Imasaka, T
为了评价光学色谱的性能，使用射线光学模型从理论上推导出一些方程。这些数学形式主义的实验验证，通过确定之间的关系的运动的聚苯乙烯珠的速度相对于所施加的辐射力的强度的条件下，不存在施加的流体现在。确认力在焦点处最大，并且随着距该位置的距离增加而减小。当粒子直径远小于光束直径时，辐射力与粒子尺寸的平方成正比。此外，辐射力被确定为与激光功率成正比。这些结果与所提出的基于射线光学的理论模型非常吻合。此外，通过与常规色谱法类比，计算了保留距离、选择性、理论塔板数和分辨率等基本参数，并讨论了色谱分离的最佳条件。结果表明，提高激光功率和降低电流速率可以扩大动态范围。峰值展宽主要是由激光功率的变化引起的，现在是由大颗粒（> 1 μ m）的介质速率引起的。理论上，对于直径大于1 μ m的颗粒，可以区分直径相差小于1%的颗粒。在20 μ m·s ~（-1）的流速和700 mW的激光功率下，三种尺寸的聚苯乙烯珠得到了很好的分离。该技术也适用于人红细胞的分离。观察到两种组分，一种由直径为1.5至2.4 μ m的细胞组成，另一种由直径为3.5至5.7 μ m的细胞组成。光学色谱可用于颗粒和生物细胞的分离和尺寸测量。
To evaluate the performance of optical chromatography, a number of equations are theoretically derived using a ray-optics model. These mathematical formalisms are experimentally verified by determining the relationship between the velocity of motion of a polystyrene bead with respect to the intensity of an applied radiation force under the condition where there exists no applied fluid now. The force is confirmed to be at a maximum at the focal point and to decrease with increasing distance from this position. The radiation force is verified to be proportional to the square of the particle size when the particle diameter is much smaller than the beam diameter. In addition, the radiation force is ascertained to be proportional to the laser power. These results are in excellent agreement with the proposed theoretical model, which is based on ray optics. Furthermore, by analogy with conventional chromatography, fundamental parameters such as retention distance, selectivity, theoretical plate number, and resolution are calculated, and optimum conditions for chromatographic separation are discussed. Based on the results obtained, the dynamic range can be extended by increasing laser power and decreasing now rate. Peak broadening is primarily caused by variations in laser power and now rate of the medium for large particles (> 1 mu m). It is possible, in theory, to distinguish particles whose diameters differ by less than 1% for particles with a diameter larger than 1 mu m. Three sizes of polystyrene beads are well separated at a now rate of 20 mu m s(-1) and a laser power of 700 mW. This technique is also applied to the separation of human erythrocytes. Two fractions, one consisting of cells ranging from 1.5 to 2.4 mu m in diameter and another consisting of cells ranging from 3.5 to 5.7 mu m in diameter, are observed. Optical chromatography is useful for separation and size measurement of particles and biological cells.