A transcriptomic landscape of human papillomavirus 16 E6‐regulated gene expression and splicing events
A transcriptomic landscape of human papillomavirus 16 E6‐regulated gene expression and splicing events
复制标题
DOI：
10.1002/1873-3468.12486
复制
发表时间：
2016-12
期刊：
影响因子：
3.5
通讯作者：
Junfen Xu;Yifeng Fang;J. Qin;XiaoJing Chen;X. Liang;Xing Xie;Weiguo Lu
中科院分区：
文献类型：
--
作者：
Junfen Xu;Yifeng Fang;J. Qin;XiaoJing Chen;X. Liang;Xing Xie;Weiguo Lu
高危型人乳头瘤病毒（HPV）的癌蛋白E6是宫颈癌发生和进展的原因。在本研究中，我们对表达HPV16 E6的293T细胞进行了RNA测序分析，确定了56个差异表达基因（DEG）以及许多受到显著影响的细胞通路。我们在细胞模型和临床组织样本中证实了其中9个差异表达基因。此外，我们对1128个新的人类转录本进行了从头转录组组装，并确定了22个在HPV16 E6存在时差异表达的转录本。另外，我们的分析揭示了响应HPV16 E6表达的不同可变剪接事件。总体而言，本研究提供了转录和剪接特征的全面描述，以及响应HPV16 E6差异表达的先前未知基因，这促使需要对HPV16 E6调控的转录组进行完整注释。
The oncoprotein E6 of high‐risk human papillomavirus (HPV) is responsible for the initiation and progression of cervical cancer. In the present study, we performed RNA‐sequencing analysis in HPV16 E6‐expressing 293T cells and identified 56 differentially expressed genes (DEGs) and a number of cellular pathways significantly affected. We confirmed nine of these DEGs in both cell models and clinical tissue samples. Furthermore, we performed de novo transcriptome assembly of 1128 novel human transcripts and identified 22 that are differentially expressed in the presence of HPV16 E6. In addition, our analysis revealed distinct alternative splicing events in response to HPV16 E6 expression. Overall, the present study provides a comprehensive portrait of transcriptional and splicing signatures, as well as previously unknown genes differentially expressed in response to HPV16 E6, which prompts the need for a complete annotation of the HPV16 E6‐regulated transcriptome.