INTERLEUKIN-1 ACTIVITY IN THE SYNOVIAL-FLUID OF PATIENTS WITH RHEUMATOID-ARTHRITIS
INTERLEUKIN-1 ACTIVITY IN THE SYNOVIAL-FLUID OF PATIENTS WITH RHEUMATOID-ARTHRITIS
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DOI：
10.1007/bf00541245
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发表时间：
1982-01-01
影响因子：
4
通讯作者：
COHEN, G
中科院分区：
文献类型：
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作者：
FONTANA, A;HENGARTNER, H;COHEN, G
本文观察了类风湿关节炎（RA）患者滑液（SF）对C_3 H/HeJ小鼠胸腺细胞的影响。在20个SF测试，17（85%）表现出增强的植物血凝素（PHA）诱导的胸腺细胞刺激。在16例骨关节病患者的SF中，仅在1例（6.25%）中检测到这种活动。对扩增因子的进一步表征表明：（1）RA患者的SF增强了胸腺细胞对PHA和伴刀豆球蛋白A的反应;（2）在无丝裂原的情况下，SF处理的胸腺细胞显示出对~3H-胸腺嘧啶核苷的摄取增加;（3）SF不促进白细胞介素2依赖性卵清蛋白特异性T细胞克隆的生长，但（4）SF被发现是次适剂量凝集素刺激脾细胞释放最佳白细胞介素2所必需的。基于这些生物学效应，RA患者SF中的因子被认为是白细胞介素1（IL-1）。在培养物中由活化的巨噬细胞产生的IL-1已显示刺激T和B细胞功能，并诱导培养的滑膜细胞产生胶原酶和胡萝卜素。IL-1的这两种特性可能与RA的发病机制有关。
The synovial fluids (SF) of patients with rheumatoid arthritis (RA) were investigated for their effects on thymocytes of C3H/HeJ mice. Of the 20 SF tested, 17 (85%) showed an augmentation of the phytohaemagglutinin (PHA) induced thymocyte stimulation. Out of 16 SF of patients with osteoarthrosis, such an activity was detected in only one (6.25%). Further characterisation of the amplification factor revealed that (1) the SF of RA patients augmented both the PHA and the Concanavalin A response of the thymocytes (2) in the absence of mitogens, SF-treated thymocytes showed an increased uptake of3H-thymidine, (3) the SF did not propagate the growth of an interleukin 2 dependent ovalbumin specific T cell clone, but (4) the SF were found to be required for optimal interleukin 2 release by spleen cells stimulated with suboptimal doses of lectin. Based on these biological effects the factor in the SF of RA patients is suggested to represent an interleukin 1 (IL-1). IL-1 produced in cultures by activated macrophages has been shown to stimulate T and B cell functions and to induce the production of collagenase and prostaglandins by cultured synovial cells. Both properties of IL-1 could be relevant in the pathogenesis of RA.