Peripheral antioxidant enzyme activities and selenium in elderly subjects and in dementia of Alzheimer's type - Place of the extracellular glutathione peroxidase
Peripheral antioxidant enzyme activities and selenium in elderly subjects and in dementia of Alzheimer's type - Place of the extracellular glutathione peroxidase
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DOI：
10.1016/0891-5849(95)02058-6
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发表时间：
1996-01-01
影响因子：
7.4
通讯作者：
Berr, C
中科院分区：
文献类型：
--
作者：
CeballosPicot, I;MeradBoudia, M;Berr, C
对40例阿尔茨海默型痴呆（DAT）患者和34例认知功能正常的老年对照者的红细胞和血浆进行了抗自由基损伤的测定。DAT患者红细胞铜锌超氧化物歧化酶（E-CuZnSOD）、硒依赖型谷胱甘肽过氧化物酶（E-GSH-Px）、谷胱甘肽还原酶（E-GSSG-RD）活性和硒（Se）浓度与对照组无显著性差异。与对照组相比，DAT组血浆中通过硫代巴比妥反应物质（TEARS）评价的过氧化产物处于相同水平。DAT组血浆GSH-Px（P-GSH-Px）活性和血浆Se（P-Se）与年龄呈负相关（分别为r = -0.58，p < 0.001和r = -0.63，p < 0.001）。红细胞GSH-Px活性和Se与年龄呈负相关（分别为r = -0.40; p < 0.01和r = -0.46; p < 0.01）。在对照组中未观察到与年龄的显著相关性。当控制年龄时，DAT患者的P-GSH-Px活性和P-Se显著高于对照组。这些显著性差异主要出现在80岁以下的DAT受试者中。仅在DAT组中观察到一些相关性，如P-GSH-Px和E-GSH-Px（r = +0.68; p < 0.001）; P-GSH-Px和E-Se（r = +0.79; p < 0.001）。DAT组P-GSH-Px与P-Se、E-GSH-Pr与P-Se、P-Se与E-Se的相关性较强（r = +0.84; p < 0.001; r =+0.76; p < 0.001和r = 0.75; p < 0.001）（r = 0.54，pI < 0.01; r = 0.43，p < 0.01和r =+0.34，p < 0.05）。事实上，第一，一个显着增加P-GSH-Px和P-Se，第二，一些抗氧化参数之间的关系的修改，和第三年龄依赖性降低谷胱甘肽过氧化物酶的活动和他们的辅因子，出现在DAT组表明，DAT是与氧化应激由于活性氧和外周抗氧化剂之间的不平衡的对立力量。
Defenses against free radical damage were determined in red blood cells and plasma from 40 patients with dementia of the Alzheimer-type (DAT) and 34 aged control subjects with normal cognitive function. No crude significant difference in erythrocyte copper-zinc superoxide dismutase (E-CuZnSOD), seleno-dependent glutathione peroxidase (E-GSH-Px), glutathione reductase (E-GSSG-RD) activities, and selenium (Se) concentration was found between DAT cases and control subjects. The peroxidation products evaluated in plasma by the thiobarbituric-reactive material (TEARS) were at the same level in the DAT group as compared to controls. In the DAT group, plasma GSH-Px (P-GSH-Px) activity and plasma Se (P-Se) were negatively correlated with age (r = -0.58; p < 0.001 and r = -0.63; p < 0.001 respectively). Moreover, erythrocyte GSH-Px activity and Se were also negatively correlated with age (r = -0.40; p < 0.01 and r = -0.46; p < 0.01, respectively). No significant correlation with age was observed in the controls. When controlling for age, a significant increase for P-GSH-Px activity and P-Se was observed in DAT patients as compared to controls. These significant differences mostly appeared in DAT subjects under 80 years. Some correlations were only observed in the DAT group such as P-GSH-Px and E-GSH-Px (r = +0.68; p < 0.001); P-GSH-Px and E-Se (r = +0.79; p < 0.001). Correlations between P-GSH-Px and P-Se, E-GSH Pr and P-Se, and P-Se with E-Se are greater in the DAT group (r = +0.84; p < 0.001; r +0.76; p < 0.001 and r 0.75; p < 0.001) than in the control group (r = 0.54, pI < 0.01; r = 0.43, p < 0.01 and r = +0.34, p < 0.05 respectively). The fact that first-a significant increase in P-GSH-Px and P-Se, second-some modifications in the relationships between antioxidant parameters, and third-age-dependent decreases of glutathione-peroxidase activities and their cofactor, appeared only in the DAT group suggest that DAT is associated with an oxidative stress due to an imbalance between reactive oxygen species and the peripheral antioxidant opposing forces.