The heme auxotroph Caenorhabditis elegans can cleave the thioether bonds of c-type cytochromes.
The heme auxotroph Caenorhabditis elegans can cleave the thioether bonds of c-type cytochromes.
复制标题
血红素营养缺陷型秀丽隐杆线虫可以裂解 c 型细胞色素的硫醚键。
DOI：
10.1002/1873-3468.13006
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Murphey AC
中科院分区：
文献类型：
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作者：
Murphey AC
血红素是必不可少的，通过高度调节的过程合成。出于这个原因，大多数生物都努力回收它或从环境中获取它。当血红素与蛋白质非共价结合时，多肽的降解足以释放它。然而，在某些血红素蛋白中，例如c-型细胞色素，血红素与蛋白质骨架共价结合。我们使用血红素营养缺陷型秀丽隐杆线虫来研究细胞色素c是否可以是血红素来源，我们表明这种生物体必须编码一种新的系统，该系统特异性地切割c型细胞色素的硫醚键。我们还发现，在有限的血红素浓度下，虽然体细胞组织正常发育，但生殖细胞无法增殖，这表明C中存在血红素敏感检查点。优美的
Heme is essential and synthesized via highly regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins noncovalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such asc‐type cytochromes, heme is covalently bound to the protein backbone. We use the heme auxotrophCaenorhabditis elegansto investigate if cytochromesccan be a heme source, and we show that this organism must encode a novel system which specifically cleaves the thioether bonds ofc‐type cytochromes. We also find that at limiting heme concentrations, while somatic tissues develop normally the germline fails to proliferate, suggesting the presence of a heme‐sensing checkpoint inC. elegans.