Cocaine-induced dopamine overflow within the nucleus accumbens measured by in vivo microdialysis: A meta-analysis
Cocaine-induced dopamine overflow within the nucleus accumbens measured by in vivo microdialysis: A meta-analysis
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DOI：
10.1002/syn.20489
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发表时间：
2008-04-01
期刊：
影响因子：
2.3
通讯作者：
Spanagel, Rainer
中科院分区：
文献类型：
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作者：
Frank, Stefanie Th.;Krumm, Bertram;Spanagel, Rainer
Meta分析来自已发表的使用体内微透析评估可卡因对伏隔核(NAC)内多巴胺(DA)溢出的剂量-反应曲线的文章。考虑了不同的实验和生物学参数，如给药途径(ip，sc，iv，local)，大鼠和小鼠品系，性别，年龄方面，以及管状区域(NAC核心和外壳)。来自116个实验的833只动物的数据符合我们的选择标准，并分析了绝对基础DA水平、DA溢出的最大峰值(峰值[%]基线)和该峰值出现的时间(峰值时间)。我们的荟萃分析显示，DA的绝对基础水平位于2.39 NM(所有实验的中位数)，并且可卡因诱导的NAC中DA溢出在所应用的剂量范围内以线性剂量-反应方式显著增强，随着静脉或ip给药后回归函数的增加。静脉注射时达峰时间最快，局部用药后最慢。此外，激电实验表明，剂量越高，达到顶峰所需的时间越长。来自NAC壳区的结果显示，与NAC核心相比，DA溢出更大。可卡因处理后小鼠和大鼠的DA溢出特性没有不同。因此，无论是物种差异还是其他生物因素，如年龄、性别和大鼠/小鼠品系，都不会对可卡因诱导的DA溢出产生显著影响。微透析程序的技术参数，如灌流介质的钙浓度和采集的样本量，也不会对可卡因治疗后DA溢出特性(峰[%]、基线和峰时间)产生显著影响。总之，这些数据可被认为对教科书知识和通过概括现有数据提供的数据更好的可比性以及对调查人员最大限度地发挥可卡因诱导的DA溢出的影响是有用的。最后，这项研究举例说明了如何将荟萃分析应用于神经化学领域内的广泛数据。
A meta-analysis was conducted on data obtained from published articles which used in vivo microdialysis to assess dose-response curves of cocaine on dopamine (DA) overflow within the nucleus accumbens (NAC). Different experimental and biological parameters such as route of administration (ip, sc, iv, local), rat and mouse strains, gender, age aspects, and regions cannulated (NAC core and shell) were considered. Data from 116 experiments involving 833 animals (out of 266 publications) fulfilled our selection criteria and were analyzed in relation to absolute basal DA levels, the maximum peak of DA overflow (peak [%] baseline) and the time when this peak (peak time) occurred. Our meta-analysis revealed that absolute basal DA levels lie at 2.39 nM (median of all experiments) and that cocaine-induced DA overflow in the NAC is significantly enhanced in a linear dose-response fashion within the applied dose range as the regression function increases following either iv or ip administration. Peak time was reached fastest in iv experiments and slowest following local application. Furthermore, it was shown in ip experiments that the higher the dose, the longer it took to reach the zenith. Results from the NAC shell region displayed greater DA overflow as compared with the NAC core. DA overflow properties following cocaine treatment in mice did not differ from that in rats. Thus, neither species differences nor other biological factors such as, age, gender, and rat/mouse strain have a pronounced impact on cocaine-induced DA overflow. Technical parameters of the microdialysis procedure such as calcium concentration of the perfusion medium and collected sample amount have also no significant effect in terms of DA overflow properties (peak [%] baseline and peak time) following cocaine treatment. In conclusion, these data may be deemed useful for textbook knowledge and a better comparability of data given by the generalization of already existing data as well as for investigators in maximizing the effect of cocaine-induced DA overflow. Finally, this study examplifies how meta-analyses may be applied to a wide range of data within the field of neurochemistry.