Rapid stimulation of pinocytosis in human carcinoma cells A-431 by epidermal growth factor.
Rapid stimulation of pinocytosis in human carcinoma cells A-431 by epidermal growth factor.
复制标题
DOI：
10.1083/jcb.83.1.82
复制
发表时间：
1979-10
期刊：
影响因子：
--
通讯作者：
Cohen S
中科院分区：
文献类型：
--
作者：
Haigler HT;McKanna JA;Cohen S
辣根过氧化物酶（HRP）摄取用于测量人上皮样癌细胞（A-431）单层中的液相胞饮。组织化学证实，细胞相关的HRP仅限于胞内囊泡。生化方法表明，HRP的摄取在控制文化是成正比的曝光时间。加入低浓度的表皮生长因子（EGF）的孵育介质中产生了10倍的初始速率增加的胞饮。EGF的作用是快速的（30秒内），但短暂的，胞饮率在15分钟内恢复到对照水平。代谢抑制剂减少EGF刺激的胞饮率大于90%。EGF和铁蛋白（F：EGF）的共轭物被用来同时比较EGF和HRP的细胞内位置。大部分F：EGF内化在约100 nm的囊泡中，而大部分HRP位于更大的囊泡（范围0.1- 1.2 μ m）中，其中也含有F：EGF。肿瘤促进剂12-0- tetradecanoyl-phorbol-13-acetate与EGF具有几种生物活性，也能有效刺激胞饮速率的增加。
Horseradish peroxidase (HRP) uptake was used to measure fluid-phase pinocytosis in monolayers of human epithelioid carcinoma cells (A-431). Histochemistry confirmed that cell-associated HRP was restricted to intracellular vesicles. Biochemical methods showed that HRP uptake in control cultures was directly proportional to the duration of exposure. The addition of low concentrations of epidermal growth factor (EGF) to the incubation media produced a 10-fold increase in the initial rate of pinocytosis. The EGF effect was rapid (within 30 s) but transient; the rate of pinocytosis returned to control levels within 15 min. Metabolic inhibitors reduced the EGF-stimulated rate of pinocytosis by greater than 90%. A conjugate of EGF and ferritin (F:EGF) was used to simultaneously compare the intracellular locations of EGF and HRP. Much of F:EGF was internalized in approximately 100-nm vesicles, while most of the HRP was located in much larger vesicles (range 0.1--1.2 micrometer) which also contained F:EGF. The tumor-promoter 12-0- tetradecanoyl-phorbol-13-acetate, which shares several biological activities with EGF, was also effective in stimulating an increase in the rate of pinocytosis.