Triggering of Autophagy by Baicalein in Response to Apoptosis after Spinal Cord Injury: Possible Involvement of the PI3K Activation
Triggering of Autophagy by Baicalein in Response to Apoptosis after Spinal Cord Injury: Possible Involvement of the PI3K Activation
复制标题
脊髓损伤后黄芩素触发自噬响应细胞凋亡：可能涉及 PI3K 激活
DOI：
10.1248/bpb.b17-00768
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发表时间：
2018-04-01
影响因子：
2
通讯作者：
Mei, Xifan
中科院分区：
文献类型：
--
作者：
Li, Yuanlong;Lin, Sen;Mei, Xifan
脊髓损伤（SCI）引起运动神经元丢失和功能障碍，导致细胞高度凋亡，造成严重损害。我们前期的研究表明，抑制自噬可以激活细胞凋亡。有趣的是，黄芩素，一种具有抗凋亡活性的药物，来自草药黄芩的根，在很大程度上通过激活磷脂酰肌醇3-激酶（PI 3 K）诱导自噬。本研究观察了腹腔注射黄芩素对脊髓损伤小鼠自噬和凋亡的影响，并探讨了自噬和凋亡之间的关系。我们证明黄芩素在脊髓损伤后7天促进运动神经元的功能恢复。此外，黄芩素增强神经元自噬和自噬相关因子PI 3 K，同时抑制p62蛋白。黄芩素治疗可减少脊髓损伤后7 d神经细胞凋亡。此外，在抑制自噬的同时，黄芩素处理损伤后细胞凋亡增加。因此，黄芩素可能通过激活PI 3 K诱导自噬以减少神经元凋亡来减轻SCI。
High level apoptosis induced by spinal cord injury (SCI) evokes serious damage because of the loss and dysfunction of motor neurons. Our previous studies showed that inhibition of autophagy evokes the activation of apoptosis. Interestingly, Baicalein, a medicine with anti-apoptosis activity that is derived from the roots of herb Scutellaria baicalensis, largely induces autophagy by activating phosphatidylinositol 3-kinase (PI3K). In this study, we investigated the effects of intraperitoneal injection of Baicalein on autophagy and apoptosis in SCI mice and evaluated the relationship between autophagy and apoptosis. We demonstrated that Baicalein promoted the functional recovery of motor neurons at 7d after SCI. In addition, Baicalein enhanced neuronal autophagy and the autophagy-related factor PI3K, while inhibiting the p62 protein. Baicalein treatment decreased neuronal apoptosis at 7d after SCI. Moreover, when inhibiting autophagy, apoptosis was upgraded by Baicalein treatment after injury. Thus, Baicalein attenuated SCI by inducing autophagy to reduce apoptosis in neurons potentially via activating PI3K.