Toward an RNaseA mimic: a DNAzyme with imidazoles and cationic amines
Toward an RNaseA mimic: a DNAzyme with imidazoles and cationic amines
复制标题
DOI：
10.1021/ja0205075
复制
发表时间：
2002-08-28
影响因子：
15
通讯作者：
Perrin, DM
中科院分区：
文献类型：
--
作者：
Lermer, L;Roupioz, Y;Perrin, DM
位点特异性RNA切割近年来受到了相当大的关注。将咪唑或胺或两者附加到寡核苷酸上以靶向特异性RNA切割的定向合成代表了令人兴奋的研究途径。然而，迄今为止，这种合成构建体的催化作用，特别是在周转方面，一直难以观察到。这是第一次报告的一个真正的催化M2+-独立的DNA酶合成修饰咪唑和阳离子胺，似乎模仿RNaseA。这项工作现在展示了合成有机化学与组合选择相结合时，如何产生一类新的DNA酶，以满足开发相对较小的仿生催化剂的持续合成挑战。
Site-specific RNA cleavage has received considerable attention over the years. Directed synthesis to append imidazoles or amines or both to oligonucleotides to target specific RNA cleavage represents an exciting avenue of research. However, to date catalysis by such synthetic constructs, particularly in terms of turnover, has been difficult to observe. This is the first report of a truly catalytic M2+-independent DNAzyme synthetically modified with imidazoles and cationic amines that would seem to mimic RNaseA. This work now demonstrates how synthetic organic chemistry, when merged with combinatorial selection, can result in a new class of DNAzymes that meets the ongoing synthetic challenges for developing relatively small biomimetic catalysts.