LYME-DISEASE SPIROCHETES AND IXODID TICK SPIROCHETES SHARE A COMMON SURFACE ANTIGENIC DETERMINANT DEFINED BY A MONOCLONAL-ANTIBODY
LYME-DISEASE SPIROCHETES AND IXODID TICK SPIROCHETES SHARE A COMMON SURFACE ANTIGENIC DETERMINANT DEFINED BY A MONOCLONAL-ANTIBODY
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DOI：
10.1128/iai.41.2.795-804.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
TODD, WJ
中科院分区：
文献类型：
--
作者：
BARBOUR, AG;TESSIER, SL;TODD, WJ
培养了一种小鼠单克隆抗体（H5332），用于抵抗从大蜱蜱中分离的螺旋体B31菌株。在间接免疫荧光分析和western blot分析中，H5332与从蓖麻蜱、田鼠、浣熊和莱姆病患者中分离的B31菌株和螺旋体的全细胞或分离组分发生反应。H5332不与代表性的螺旋体、密螺旋体和钩体结合。利用间接免疫荧光法和免疫电镜，发现H5332决定因子弥散分布在螺旋体表面，但在固定前与H5332和第二配体孵养时聚集成斑块。放射免疫沉淀和western blot研究显示，H5332决定因子位于丰富的外膜蛋白上或与之紧密相关，亚基分子量为31,000。
A murine monoclonal antibody (H5332) was raised against a spirochete, strain B31, isolated from Ixodes dammini ticks. In indirect immunofluorescence assays and western blot analyses, H5332 reacted with whole cells or isolated components of strain B31 and of spirochetes isolated from I. ricinus ticks, a field mouse, a raccoon and patients with Lyme disease. H5332 did not bind to representative borreliae, treponemes and leptospires. Using indirect immunofluorescence assays and immune EM, the H5332 determinant was found to be diffusely distributed over the surface of prefixed spirochetes but aggregated in patches when the organisms were incubated with H5332 and a 2nd ligand before fixation. Radioimmunoprecipitation and western blot studies revealed the H5332 determinant to be on or tightly associated with an abundant outer membrane protein with an apparent subunit MW of 31,000.