The feasibility of immobilized Chlorella vulgaris cultivated in palm oil mill effluent for lipid and fatty acid methyl ester production
The feasibility of immobilized Chlorella vulgaris cultivated in palm oil mill effluent for lipid and fatty acid methyl ester production
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DOI：
10.1016/j.matpr.2021.09.373
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发表时间：
2021-10
期刊：
影响因子：
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通讯作者：
N. R. Abu Sepian;N. H. Mat Yasin;N. Zainol
中科院分区：
文献类型：
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作者：
N. R. Abu Sepian;N. H. Mat Yasin;N. Zainol
马来西亚棕榈油厂流出物（POME）的丰富为科学家提供了一个机会，可以用微藻培养过程取代普通的培养物。然而，由于微藻体积小，给其与培养基的分离带来了困难。因此，本研究采用固定化技术简化了普通小球藻的分离过程。一个新的固定式。本实验采用海藻酸钠（SA）、海藻酸钙（CA）和羧甲基纤维素钠（CMC）组合基质，体积比（基质：微藻）为0.3:1。通过对固定化c的培养，确定了POME作为培养基的可行性。不同浓度的POME（20-100%）。本研究探索了两种培养基：POME与蒸馏水混合（DW）（0-80%）和POME与Bold基础培养基（BBM）混合（0-80%）。从细胞密度结果来看，20% POME + 80% DW和40% POME + 60% BBM培养基的细胞数最高，分别为1.63 × 109cells/mL和0.59 × 109cells/mL。同时，POME混合培养基显示出作为固定化微藻培养基的潜力。在BBM培养基中固定化微藻珠提取的脂质最高（48.03±3.7%），其次为20% POME + 80% DW(38.14±2.2%)，最低为40% POME + 60% BBM（32.93±2.8%）。此外，固定化微藻生物质在POME混合培养基中提取的油脂制备的FAME具有良好的油脂品质。因此，产生的油显示了生产生物柴油的潜力。
The abundance of palm oil mill effluent (POME) in Malaysia had exposed an opportunity for the researchers to replace the common culture for microalgae cultivation process. However, the small size of microalgae bring difficulties in the separation process from the culture medium. Thus, immobilization technique had been implemented in this study to simplify the separation process of microalgae Chlorella vulgaris. A new immobilizedC. vulgarisconsisted of combination matrix (sodium alginate (SA),calcium alginate (CA) and sodium carboxymethyl cellulose (CMC)) with volumetric ratio (Matrix:Microalgae) 0.3:1 were utilized in this experiment. The feasibility of POME as a cultivation medium was determined by cultivating the immobilizedC. vulgarisin different concentration of POME (20–100%). Two types of media were explored in this study which were POME mixed with distilled water (DW) (0–80%) and POME mixed with Bold Basal Medium (BBM) (0–80%). Based on the cell density result, the medium with 20% POME + 80% DW and 40% POME + 60% BBM medium performed the highest number of cells with 1.63 × 109cells/mL and 0.59 × 109cells/mL, respectively. Meanwhile, the the POME mixture medium showed the potential as a cultivation medium for the immobilized microalgae. The lipid extracted from immobilized microalgae beads cultivated in BBM media were the highest with 48.03 ± 3.7%, followed by 20% POME + 80% DW (38.14 ± 2.2%) and the lowest by 40% POME + 60% BBM media (32.93 ± 2.8%). Besides, the FAME produced from oil extracted using immobilized microalgae biomass cultured in POME mixture medium showed a good quality of oil. Thus, the oil produced revealed the potential for biodiesel production.