Complete genome sequencing of dengue virus type 1 isolated in Buenos Aires, Argentina
Complete genome sequencing of dengue virus type 1 isolated in Buenos Aires, Argentina
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DOI：
10.1016/j.virusres.2003.12.033
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发表时间：
2004-05-01
期刊：
影响因子：
5
通讯作者：
Mistchenko, AS
中科院分区：
文献类型：
--
作者：
Barrero, PR;Mistchenko, AS
登革热（DEN）是一种主要的病毒性节肢动物传播的人类疾病。南美洲上一次被认为没有登革热是在20年前，当时报告的登革热和出血性登革热病例数量急剧增加。1999-2000年巴拉圭疫情在布宜诺斯艾利斯市分离出五种病毒。将直接从血浆中获得的rt - pcr克隆到pGemT载体上，分析结构基因和NS1的序列。从细胞培养分离物中获得三种病毒的RT-PCR全长测序。同义突变多于非同义突变表明，结构蛋白受到强烈的功能限制，而整个多蛋白中存在弱的纯化选择。序列多样性和选择压力因患者而异，但结果明显高于手术阈值。一个样本显示小斑块表型和生长受损，与3'非翻译区突变有关。全长序列的系统发育分析将布宜诺斯艾利斯分离株划分为美洲den1基因型V的两个聚类：进化枝I与巴西样本系统发育相关，进化枝11与巴拉圭和阿根廷东北部样本系统发育相关。在布宜诺斯艾利斯市，虽然没有发现本地传播的证据，但只发现了从巴拉圭输入的血清型登革热病毒。(C) 2004 Elsevier B.V.版权所有
Dengue (DEN) constitutes a major viral arthropod-borne human illness. South America was last considered free of dengue two decades ago when a dramatic increase in the number of dengue fever and hemorrhagic dengue cases had been reported. Five viruses were isolated in Buenos Aires City from the 1999-2000 Paraguay outbreak. RT-PCRs obtained directly from plasma were cloned into pGemT vectors and sequences of the structural genes and NS1 were analyzed. Three viruses were full-length sequenced from RT-PCR obtained from cell-culture isolates. Excess of synonymous over non-synonymous mutations suggested that the structural proteins were under strong functional constraints while a weak purifying selection was operating in the whole polyprotein. Sequence diversity and selective pressures varied among patients but results were significantly above the procedure threshold. One sample showed small-plaque phenotype and impaired growth coupled to 3' untranslated region mutations. Phylogenetic analysis of full-length sequences split Buenos Aires isolates into two clusters within American DEN-1 genotype V: Clade I was phylogenetically linked to Brazilian samples and Clade 11 with samples from Paraguay and Northeastern Argentina. In Buenos Aires City, only dengue vir-us serotype I imported from Paraguay has been detected, though without evidence of local transmission. (C) 2004 Elsevier B.V. All rights reserved.