Capillaries modified by polyelectrolyte multilayers for electrophoretic separations
Capillaries modified by polyelectrolyte multilayers for electrophoretic separations
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DOI：
10.1021/ac990277l
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发表时间：
1999-09-15
影响因子：
7.4
通讯作者：
Schlenoff, JB
中科院分区：
文献类型：
--
作者：
Graul, TW;Schlenoff, JB
涂有物理吸附的带电聚合物薄膜的熔融二氧化硅毛细管用于毛细管区带电泳分离。该涂层是一种多层结构，通过用带正电荷和负电荷的聚合物交替冲洗而原位构建。多层膜的厚度和表面电荷量由沉积溶液中盐的浓度控制。电渗透的方向现在随着多层表面电荷在积聚期间极性的交替而振荡。的表观表面电荷，推导出的电渗流动性，是远远小于标称的表面电荷的膜。机制限制这种表观表面电荷的缓冲渗透层进行了讨论。多层涂层的列表现出许多理想的功能，除了易于建设和可重现的控制电渗流：稳定的现在的速度是立即实现对柱暴露于运行缓冲液，现在逆转是可能的。还发现色谱柱对极端pH值和离子强度以及脱水/再水化是稳定的。一系列的碱性蛋白质被分离，具有良好的效率，证明柱不可逆的蛋白质吸附的阻力。使用较厚的膜的中性溶质的分配和分离被证明。
Fused silica capillaries coated with thin films of physically adsorbed charged polymers are employed for capillary zone electrophoretic separations. The coating is a polyelectrolyte multilayer,constructed in situ by alternating rinses with positively and negatively charged polymers. The thickness of the multilayer and amount of surface charge is controlled by the concentration of salt in the deposition solutions. The direction of the electroosmotic now oscillates as the multilayer surface charge alternates in polarity during buildup. The apparent surface charge, deduced from the electroosmotic mobility, is considerably less than the nominal surface charge of the film. Mechanisms limiting this apparent surface charge for a buffer permeable layer are discussed. The multilayer-coated columns exhibit many desirable features in addition to ease of construction and reproducible control of electroosmotic flow: stable now rates are achieved immediately on exposure of the column to running buffer, and reversed now is possible. Columns are also found to be stable to extremes of pH and ionic strength, and to dehydration/rehydration. A series of basic proteins are separated with good efficiency, demonstrating column resistance to irreversible protein adsorption. Partitioning and separation of neutral solutes using thicker films is demonstrated.