Relevance of detection of immunoglobulin M antibody response in birds used for arbovirus surveillance
Relevance of detection of immunoglobulin M antibody response in birds used for arbovirus surveillance
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用于虫媒病毒监测的鸟类免疫球蛋白 M 抗体反应检测的相关性
DOI：
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发表时间：
1986
影响因子：
9.4
通讯作者：
M. I. Mahmud
中科院分区：
文献类型：
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作者：
C. Calisher;H. N. Fremount;W. L. Vesely;A. el;M. I. Mahmud
用5，000 PFU的东部马脑炎（EAE）病毒接种幼龄鸡，然后间隔流血的，测定血凝抑制（HI）、中和（N）、免疫球蛋白M（IgM）和IgG抗体。HI、N、IgM抗体在感染后4天首次检测，IgG在感染后7天检测。所有四种抗体在感染后持续至第90天。HI、N和IgM抗体滴度保持升高，并且与相关甲病毒西部马脑炎（WEE）病毒没有交叉反应。IgG抗体滴度也保持较高，但异源反应WEE病毒感染后随着时间的推移而增加。从哨兵鸡和野生鸟类的血清样品感染的自然界与ESTA，WEE，或圣路易斯脑炎病毒，并提交给本实验室从国家和地方卫生部门进行了IgM抗体检测，通过使用抗鸡IgM抗体捕获和IgG抗体的ESTA和WEE病毒。HI、N和IgM（表明近期感染）或IgG（表明更久远感染）抗体之间基本上完全相关。我们的结论是，IgM抗体捕获酶免疫测定法可作为一种特异性和敏感性的测定，以取代常规使用的HI试验检测抗体的哨兵鸡和年轻的，野生鸟类用于虫媒病毒监测。该检测快速且相对便宜，基本上可在所有供应充足的实验室进行。
Young chickens were inoculated with 5,000 PFU of eastern equine encephalitis (EEE) virus and bled at intervals thereafter for determinations of hemagglutination-inhibiting (HI), neutralizing (N), immunoglobulin M (IgM), and IgG antibodies. HI, N, and IgM antibodies were first detected 4 days after infection, and IgG was detected 7 days after infection. All four antibodies persisted through day 90 after infection. HI, N, and IgM antibody titers remained elevated and were not cross-reactive with the related alphavirus western equine encephalitis (WEE) virus. IgG antibody titers also remained high, but heterologous reactivity to WEE virus increased with time after infection. Serum samples from sentinel chickens and wild birds infected in nature with EEE, WEE, or St. Louis encephalitis virus and submitted to this laboratory from state and local health departments were tested for IgM antibody by using anti-chicken IgM for capture and for IgG antibodies to the EEE and WEE viruses. There was essentially complete correlation between HI, N, and either IgM (indicating recent infections) or IgG (indicating more remote infections) antibody. We conclude that the IgM antibody capture enzyme immunoassay can be used as a specific and sensitive assay to replace the routinely used HI test for detecting antibody in sentinel chickens and in young, wild birds used for arbovirus surveillance. The test is rapid and relatively inexpensive and can be performed in essentially all adequately supplied laboratories.