Diversity of Gut Microbiota Affecting Serum Level of Undercarboxylated Osteocalcin in Patients with Crohn’s Disease
Diversity of Gut Microbiota Affecting Serum Level of Undercarboxylated Osteocalcin in Patients with Crohn’s Disease
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DOI：
10.3390/nu11071541
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发表时间：
2019-07
期刊：
影响因子：
5.9
通讯作者：
Kohei Wagatsuma;S. Yamada;M. Ao;M. Matsuura;Hidemi Tsuji;T. Iida;K. Miyamoto;K. Oka;Motomichi Takahashi;Kiyoshi Tanaka;H. Nakase
中科院分区：
文献类型：
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作者：
Kohei Wagatsuma;S. Yamada;M. Ao;M. Matsuura;Hidemi Tsuji;T. Iida;K. Miyamoto;K. Oka;Motomichi Takahashi;Kiyoshi Tanaka;H. Nakase
一些报告指出，血浆维生素K水平下降与骨密度之间可能存在联系。有人认为肠道细菌有助于维持维生素K。克罗恩病（CD）患者维生素K的减少涉及几个因素。我们旨在评估CD患者肠道微生物群与维生素K缺乏替代指标之间的关系。我们收集了26名临床非活动性CD患者的粪便。我们从粪便中的肠道细菌中提取16S rRNA，并通过聚合酶链反应对其进行扩增。通过Illumina Miseq平台使用16S宏基因组方法分析产生的聚合酶链反应产物。血清低羧化骨钙素浓度被用作维生素K缺乏的替代指标。在低活动性病例中，血清低羧化骨钙素与平均Chao1指数呈显著负相关。与维生素K正常组相比，维生素K缺乏组的肠道微生物群的多样性显著降低，瘤胃球菌科和毛螺菌科显著减少。总之，这些数据表明，即使在临床非活动性CD患者中研究肠道微生物群对改善患者的维生素K状态也具有重要意义。
Several reports have indicated a possible link between decreasing plasma levels of vitamin K and bone mineral density. It has been suggested that intestinal bacteria contribute to maintenance of vitamin K. Several factors are involved in the reduction of vitamin K in patients with Crohn’s disease (CD). We aimed to assess the relationship between gut microbiota and alternative indicators of vitamin K deficiency in patients with CD. We collected the feces of 26 patients with clinically inactive CD. We extracted 16S rRNA from the intestinal bacteria in the feces and amplified it by polymerase chain reaction. The generated polymerase chain reaction product was analyzed using a 16S metagenomic approach by Illumina Miseq platform. Serum undercarboxylated osteocalcin concentration was used as an alternative indicator of vitamin K deficiency. There was a significant negative correlation between serum undercarboxylated osteocalcin and mean Chao1 index in cases of low activity. The diversity of the gut microbiota was significantly lower, and Ruminococcaceae and Lachnospiraceae were significantly decreased in the vitamin K-deficient group in comparison to the vitamin K-normal group. Taken together, these data suggested the significance of investigating the gut microbiota even in patients with clinically inactive CD for improving patients’ vitamin K status.