Identification of terpenoids volatilized from Thymus vulgaris L. by heat treatment and their in vitro antimicrobial activity
Identification of terpenoids volatilized from Thymus vulgaris L. by heat treatment and their in vitro antimicrobial activity
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DOI：
10.1016/j.pmpp.2016.05.004
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发表时间：
2016-04-01
影响因子：
2.7
通讯作者：
Sato, Tatsuo
中科院分区：
文献类型：
--
作者：
Eguchi, Yumi;Widiastuti, Ani;Sato, Tatsuo
50 ℃ 20 s热水处理对灰葡萄孢的抗真菌活性增强，在百里香（Thumbervulgaris L.）百里香酚、对伞花烃和γ-松油烯是百里香植物中的主要挥发性化合物。处理后百里香对伞花烃和γ-萜品烯的释放量增加，而百里香酚的释放量没有变化，表明对伞花烃和γ-萜品烯的热依赖性合成途径的存在。挥发性对伞花烃在0.50 μ L/ml时抑制病原菌的菌丝生长，而γ-萜品烯在0.05、0.10和0.50 μ L/ml时具有抑制作用。γ-萜品烯基因转录水平在处理24 h后增加。结果表明，热处理通过百里香中挥发性萜类化合物的热依赖性合成途径增强了百里香的抗真菌活性。(C)2016爱思唯尔有限公司版权所有
Enhanced antifungal activity by hot water treatment at 50 degrees C 20 s against Botrytis cinerea Pers. ex Fr., a gray mold pathogen was evaluated in thyme (Thymus vulgaris L.) Thymol, p-cymene, and gamma-terpinene were detected as the main volatile compounds in thyme plants. Emission of p-cymene and gamma-terpinene from thyme increased after treatment, whereas amount of thymol released did not change, suggested that existence of a thermo-dependent synthesis pathway of p-cymene and gamma-terpinene. Volatile p-cymene inhibited mycelial growth of pathogen at 0.50 mu L/ml, whereas gamma-terpinene had an inhibitory effect at 0.05, 0.10, and 0.50 mu L/ml. Transcript level of gamma-terpinene gene increased after 24 h of treatment. As a conclusion, heat treatment enhanced antifungal activity by volatile terpenoids synthesized by thermo-dependent synthesis pathway of them in thyme. (C) 2016 Elsevier Ltd. All rights reserved.