Lineage Analysis of Circulating Trypanosoma cruzi Parasites and Their Association with Clinical Forms of Chagas Disease in Bolivia
Lineage Analysis of Circulating Trypanosoma cruzi Parasites and Their Association with Clinical Forms of Chagas Disease in Bolivia
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DOI：
10.1371/journal.pntd.0000687
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发表时间：
2010-05-01
影响因子：
3.8
通讯作者：
Hirayama, Kenji
中科院分区：
文献类型：
--
作者：
del Puerto, Ramona;Eiki Nishizawa, Juan;Hirayama, Kenji
背景：查加斯病的病原体克氏锥虫（Trypanosoma cruzi）分为6个离散型单位（DTU）：Tc I、IIa、IIb、IIc、IId和IIe。为了评估不同DTU的相对致病性，来自三个不同临床组的慢性恰加斯病患者的血液样品被收集，以确定不同DTU的相对致病性。（不确定，心脏，巨结肠）从玻利维亚的循环寄生虫谱系进行了分析，使用小环动基体DNA多态性。2000年至2007年期间，从玻利维亚圣克鲁斯的诊所和医院送往热带国家研究中心诊断南美锥虫病的病人接受了血清学评估，心脏病学和胃肠道检查。此外，还纳入了在日本大学医院因巨结肠Chagerenia而接受结肠切除术的患者。共306例慢性恰加斯病患者按其临床类型进行了定义（81例心脏病，150例无心脏病，100例巨结肠，144例无巨结肠，164例心脏病或巨结肠，73例不确定，17例心脏病和巨结肠）。从10 ml外周静脉血中提取DNA用于PCR分析。从306份样品中扩增出196份动基体小环DNA（kDNA）（64.1%），其中104 TcIId占53.3%，TcI、TcIIb、TcIIe、TcI/IId、TcIIb/d、TcIIe/IId、TcIIe/IIe/在133个TcIId样品中，检测到三种不同的kDNA高变区模式：Mn（49.6%）、TPK样（48.9%）和Bug样（1.5%）。Tc类型和疾病的临床表现之间没有显着的关联。结论：没有确定的谱系或亚系与任何特定的临床表现在慢性南美锥虫病患者在玻利维亚显着相关。
Background: The causative agent of Chagas disease, Trypanosoma cruzi, is divided into 6 Discrete Typing Units (DTU): Tc I, IIa, IIb, IIc, IId and IIe. In order to assess the relative pathogenicities of different DTUs, blood samples from three different clinical groups of chronic Chagas disease patients (indeterminate, cardiac, megacolon) from Bolivia were analyzed for their circulating parasites lineages using minicircle kinetoplast DNA polymorphism.Methods and Findings: Between 2000 and 2007, patients sent to the Centro Nacional de Enfermedades Tropicales for diagnosis of Chagas from clinics and hospitals in Santa Cruz, Bolivia, were assessed by serology, cardiology and gastrointestinal examinations. Additionally, patients who underwent colonectomies due to Chagasic magacolon at the Hospital Universitario Japones were also included. A total of 306 chronic Chagas patients were defined by their clinical types (81 with cardiopathy, 150 without cardiopathy, 100 with megacolon, 144 without megacolon, 164 with cardiopathy or megacolon, 73 indeterminate and 17 cases with both cardiopathy and megacolon). DNA was extracted from 10 ml of peripheral venous blood for PCR analysis. The kinetoplast minicircle DNA (kDNA) was amplified from 196 out of 306 samples (64.1%), of which 104 (53.3%) were Tc IId, 4 (2.0%) Tc I, 7 (3.6%) Tc IIb, 1 (0.5%) Tc IIe, 26 (13.3%) Tc I/IId, 1 (0.5%) Tc I/IIb/IId, 2 (1.0%) Tc IIb/d and 51 (25.9%) were unidentified. Of the 133 Tc IId samples, three different kDNA hypervariable region patterns were detected; Mn (49.6%), TPK like (48.9%) and Bug-like (1.5%). There was no significant association between Tc types and clinical manifestations of disease.Conclusions: None of the identified lineages or sublineages was significantly associated with any particular clinical manifestations in the chronic Chagas patients in Bolivia.