A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX
A new application of Gompertz function in photohemolysis: the effect of temperature on red blood cell hemolysis photosensitized by protoporphyrin IX
复制标题
DOI：
10.1007/s11517-006-0080-y
复制
发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Al-Akhras, M.
中科院分区：
文献类型：
--
作者：
Al-Akhras, M.
原卟啉IX（PpIX）的光敏化在不同的照射温度、不同的暗孵育温度（Tin）和不同的照射时间下加速。Gompertz函数对部分光溶血率a和部分光溶血率B的适用性被认为是最合适的模型，以最小的参数和最小的误差来拟合实验数据。注意到在低照射温度5 ℃和更高的T-inc 37 ℃下用黑光照射的20 μ M PpIX的Gompertz参数a的分数比值的降低和分数比值B的增加。参数a在较低的辐照时间和较低的辐照温度下具有较高的值，这表明较长的光溶血过程和较长的t（50）。参数B的值被认为是强烈的温度依赖性，并总是增加与增加照射时间和Tinc与较低的值在较低的照射时间和较低的Tinc。在等于或高于35摄氏度的辐照温度下，红细胞裂解过程没有显著变化。同样，在T-inc 24和37摄氏度下，在较高的照射时间下，t（50）没有显着变化。因此，Gompertz分析技术适合于研究不同条件下的光溶血过程，是一种最佳拟合模型。
Photosensitization by protoporphyrin IX (PpIX) is accelerated at different irradiation temperatures, different dark incubation temperatures (Tin,) and different irradiation times. The applicability of Gompertz function to the fractional photohemolysis ratio, a and the rate of fractional photohemolysis, b is found to be the most appropriate model to fit the experimental data with minimum parameters and minimum errors. The reduction in Gompertz parameters, the fractional ratio values of a, and increase in the fractional rate values b, for 20 mu M PpIX irradiated with black light at low irradiation temperature 5 degrees C and higher T-inc 37 degrees C was noticed. The parameter a has higher values at lower irradiation time and lower irradiation temperatures which indicates a longer photohemolysis process and longer t(50). Values of the parameter b were found to be strongly temperature-dependent, and always increase with increasing irradiation time and Tinc with lower values at lower irradiation time and lower Tinc. There are no significant changes in the lysis of RBCs process at irradiation temperatures equal to or higher than 35 degrees C. Similarly, no significant change on t(50) at higher irradiation time at T-inc 24 and 37 degrees C. In conclusion, Gompertz analysis technique adapts to study the photohemolysis process at different conditions as a best-fit model.