Genome-Wide Analysis and Hormone Regulation of Chitin Deacetylases in Silkworm
Genome-Wide Analysis and Hormone Regulation of Chitin Deacetylases in Silkworm
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家蚕甲壳素脱乙酰酶的全基因组分析和激素调控
DOI：
10.3390/ijms20071679
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发表时间：
2019-04-04
影响因子：
5.6
通讯作者：
Hou, Yong
中科院分区：
文献类型：
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作者：
Zhang, Ziyu;Yan, Jiamin;Hou, Yong
甲壳素脱乙酰酶(CDA)是昆虫体内参与甲壳素代谢的一组酶，在甲壳素的蜕皮、化蛹和修饰过程中起着至关重要的作用。在本研究中，我们从家蚕(Bombyx mori，BmCDA)中鉴定了几种CDA，并研究了不同激素对其在家蚕幼虫和胚胎细胞系(BME)中表达的影响。在家蚕基因组中发现了8个编码BmCDA的基因。它们在不同的组织中表现出不同的表达模式，并根据它们在哪里表达被分为三种类型：外骨骼、消化器官和生殖器官。此外，我们还发现一些BmCDA在幼虫的蜕皮期表达上调，尤其是在幼虫的第四蜕皮期。我们还证实，BmCDA1-6在20-羟基蜕皮酮处理后不仅在幼虫中表达上调，而且在BME细胞中也表达上调。有趣的是，保幼激素类似物处理也上调了一些BmCDA的表达。几种转录因子的过表达表明，POU转录因子POUM2可能在BmCDA的表达调控中起主要作用。最后，BmCDA1和BmCDA2的沉默并没有导致异常的表型或死亡，但可能导致了家蚕化蛹的延迟。这些结果为鳞翅目昆虫甲壳素脱乙酰酶及其表达调控提供了重要信息。
Chitin deacetylases (CDAs) are a group of enzymes involved in chitin metabolism in insects; they play a critical role in molting, pupation, and the modification of chitin. In this study, we identified several CDAs in the silkworm, Bombyx mori (BmCDA), and investigated the effect of various hormones on their expression in B. mori larvae and embryo cell lines (BmE). Eight genes encoding BmCDAs were identified in the silkworm genome. They showed different expression patterns in different tissues, and were classified into three types based on where they were expressed: the exoskeleton, digestive organs, and genital organs. Moreover, we found that some BmCDAs showed upregulated expression during the molting period, especially during the fourth molting period in larvae. We also verified that the expression of BmCDA1–6 was upregulated by treatment with 20-hydroxyecdysone not only in larvae, but also in BmE cells. Interestingly, juvenile hormone analog treatment also upregulated the expression of some BmCDAs. The overexpression of several transcription factors revealed that the POU transcription factor POUM2 may play a major role in the regulation of BmCDA expression. Finally, the silencing of BmCDA1 and BmCDA2 did not lead to abnormal phenotypes or death, but may have led to delays in silkworm pupation. These results provide important information about lepidopteran insects in terms of chitin deacetylases and the regulation of their expression.