Nitrification of high strength ammonia wastewater and nitrite accumulation characteristics.
Nitrification of high strength ammonia wastewater and nitrite accumulation characteristics.
复制标题
DOI：
10.2166/wst.2003.0585
复制
发表时间：
2003-06
期刊：
影响因子：
--
通讯作者：
Dong-Jin Kim;J. S. Chang;D. I. Lee;D. Han;Ik-Keun Yoo;G. Cha
中科院分区：
文献类型：
--
作者：
Dong-Jin Kim;J. S. Chang;D. I. Lee;D. Han;Ik-Keun Yoo;G. Cha
在污水处理中，亚硝酸盐途径的生物脱氮在节约曝气成本和作为反硝化的电子供体方面具有重要意义。在自养硝化生物膜气升式生物膜反应器中进行了污水硝化和亚硝酸盐积累试验。生物膜反应器显示出几乎完全的硝化作用，并且在1.5至3.5 kg N/m3 x d的铵负荷下，大部分氧化铵以亚硝酸盐形式存在。通过选择性抑制亚硝酸盐氧化剂和限制游离氨和溶解氧来稳定地实现亚硝酸盐积累。即使在不存在游离氨对亚硝酸盐氧化剂的抑制的情况下，也可以实现稳定的100%转化为亚硝酸盐。间歇式氨氮氧化和亚硝酸盐氧化试验表明，当游离氨浓度大于0.2mgN/L时，亚硝酸盐氧化被完全抑制。然而，亚硝酸盐氧化活性恢复尽快游离氨浓度低于阈值水平时，溶解氧浓度不是限制因素。荧光原位杂交分析的冷冻切片的亚硝酸盐积累硝化生物膜表明，β-亚类的变形菌，其中氨氧化剂属于，分布在生物膜外，而α-亚类的变形菌，其中亚硝酸盐氧化剂属于，主要是在生物膜的内部。可能的是，溶解氧不足或限制在硝化生物膜的内部部分，亚硝酸盐氧化剂存在的地方，是负责完全关闭的亚硝酸盐氧化剂的活性下的游离氨抑制的情况下。
Biological nitrogen removal via the nitrite pathway in wastewater treatment is very important in saving the cost of aeration and as an electron donor for denitrification. Wastewater nitrification and nitrite accumulation were carried out in a biofilm airlift reactor with autotrophic nitrifying biofilm. The biofilm reactor showed almost complete nitrification and most of the oxidized ammonium was present as nitrite at the ammonium load of 1.5 to 3.5 kg N/m3 x d. Nitrite accumulation was stably achieved by the selective inhibition of nitrite oxidizers with free ammonia and dissolved oxygen limitation. Stable 100% conversion to nitrite could also be achieved even under the absence of free ammonia inhibition on nitrite oxidizers. Batch ammonium oxidation and nitrite oxidation with nitrite accumulating nitrifying biofilm showed that nitrite oxidation was completely inhibited when free ammonia is higher than 0.2 mg N/L. However, nitrite oxidation activity was recovered as soon as the free ammonia concentration was below the threshold level when dissolved oxygen concentration was not the limiting factor. Fluorescence in situ hybridization analysis of cryosectioned nitrite accumulating nitrifying biofilm showed that the beta-subclass of Proteobacteria, where ammonia oxidizers belong, was distributed outside the biofilm whereas the alpha-subclass of Proteobacteria, where nitrite oxidizers belong, was found mainly in the inner part of the biofilm. It is likely that dissolved oxygen deficiency or limitation in the inner part of the nitrifying biofilm, where nitrite oxidizers exist, is responsible for the complete shut down of the nitrite oxidizers activity under the absence of free ammonia inhibition.