Monoclonal antibodies distinguish identifiable neurones in the leech
Monoclonal antibodies distinguish identifiable neurones in the leech
复制标题
单克隆抗体可区分水蛭中可识别的神经元
作者：
B. Zipser;R. McKay
用水蛭神经索免疫小鼠，获得475株杂交瘤细胞，经筛选获得单克隆抗体。大多数（约300个）与水蛭神经组织反应，但只有约40个产生抗体，识别单一种类或小组细胞。对与特定神经元反应的20种抗体进行了更详细的研究，并在此进行了描述。它们包括针对已识别的感觉神经元和运动神经元以及针对许多未识别细胞的抗体。
Monoclonal antibodies were isolated by screening 475 hybridomas obtained from mice immunized with whole leech nerve cords. The majority (about 300) reacted with leech nervous tissue, but only about 40 made antibodies that identified single kinds or small sets of cells. Twenty of the antibodies which react with specific neurones were studied in greater detail and are described here. They include antibodies against identified sensory neurones and motor neurones as well as against numerous unidentified cells.