Rapid screening of cancer margins in tissue with multimodal confocal microscopy
Rapid screening of cancer margins in tissue with multimodal confocal microscopy
复制标题
DOI：
10.1016/j.jss.2012.05.059
复制
发表时间：
2012-12-01
影响因子：
2.2
通讯作者：
Rajadhyaksha, Milind
中科院分区：
文献类型：
--
作者：
Gareau, Daniel S.;Jeon, Hana;Rajadhyaksha, Milind
背景：组织病理学检查是肿瘤完整、准确切除的指导。然而，组织病理学的准备是劳动密集型的且缓慢的，导致组织的不充分取样和边缘的不完全和/或不准确切除。我们证明了潜在的实用性的多模式共聚焦镶嵌显微镜快速筛查癌边缘，直接在新鲜的手术切除，而不需要传统的嵌入，切片，或processing.Materials和方法：一个多模式共聚焦镶嵌显微镜开发图像基底细胞癌边缘在手术皮肤切除，与显示核细节的分辨率。多模式对比度是用荧光成像细胞核和反射细胞质和真皮胶原蛋白。从莫氏手术切除的基底细胞癌35成像，马赛克分析比较与相应的冷冻pathology.Results：共焦马赛克产生在约9分钟，显示组织的视野为12毫米，放大2倍。数字染色算法将黑色和白色对比度转换为紫色和粉红色，这模拟了标准组织病理学的外观。镶嵌使快速数字筛选，模仿检查的组织pathology.Conclusions：多模态共聚焦镶嵌显微镜提供了一个技术平台，有可能使实时病理在床边。成像可以作为常规组织病理学的辅助手段，以加快边缘筛查，并指导手术更完整和准确地切除癌症。(C)2012 Elsevier Inc. All rights reserved.
Background: Complete and accurate excision of cancer is guided by the examination of histopathology. However, preparation of histopathology is labor intensive and slow, leading to insufficient sampling of tissue and incomplete and/or inaccurate excision of margins. We demonstrate the potential utility of multimodal confocal mosaicing microscopy for rapid screening of cancer margins, directly in fresh surgical excisions, without the need for conventional embedding, sectioning, or processing.Materials and methods: A multimodal confocal mosaicing microscope was developed to image basal cell carcinoma margins in surgical skin excisions, with the resolution that shows nuclear detail. Multimodal contrast is with fluorescence for imaging nuclei and reflectance for cellular cytoplasm and dermal collagen. Thirty-five excisions of basal cell carcinomas from Mohs surgery were imaged, and the mosaics analyzed by comparison with the corresponding frozen pathology.Results: Confocal mosaics are produced in about 9 min, displaying tissue in fields of view of 12mm with x2 magnification. A digital staining algorithm transforms black and white contrast to purple and pink, which simulates the appearance of standard histopathology. Mosaicing enables rapid digital screening, which mimics the examination of histopathology.Conclusions: Multimodal confocal mosaicing microscopy offers a technology platform to potentially enable real-time pathology at the bedside. The imaging may serve as an adjunct to conventional histopathology to expedite screening of margins and guide surgery toward more complete and accurate excision of cancer. (C) 2012 Elsevier Inc. All rights reserved.