Thermus amyloliquefaciens sp nov., isolated from a hot spring sediment sample
Thermus amyloliquefaciens sp nov., isolated from a hot spring sediment sample
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从温泉沉积物样品中分离出解淀粉栖热菌 sp nov.
DOI：
10.1099/ijs.0.000289
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发表时间：
2015
影响因子：
2.8
通讯作者：
Li Wen-Jun
中科院分区：
文献类型：
--
作者：
Yu Tian-Tian;Ming Hong;Yao Ji-Cheng;Zhou En-Min;Park Dong-Jin;Hozzein Wael N.;Kim Chang-Jin;Wadaan Mohammed A. M.;Li Wen-Jun
从云南省大理市洱源县牛街温泉中分离到一株革兰氏染色阴性需氧菌YIM 77409T。细胞呈杆状，菌落呈黄色圆形。菌株在pH 6.0 ~ 8.0（最适pH 7.0）和50 ~ 70℃（最适pH 60 ~ 65℃）条件下生长。甲基萘醌以MK-8为主，DNA G+C含量为66.4 mol%。主要脂肪酸（bbb10 %）为iso-C15: 0和iso-C17: 0。极性脂质由一种氨基磷脂、一种磷脂和两种糖脂组成。16S rRNA基因序列分析表明，菌株YIM 77409t与热菌（Thermus scotoductusDSM 8553T）、热菌（Thermus antranikianiiDSM 12462T）、热菌（Thermus caliditerraeYIM 77925t）和热菌（Thermus tenchongensisyim 77924T）形成聚类，与t的16S rRNA基因序列相似性最高。scotoductusDSM 8553T（97.57%）。然而，DNA-DNA杂交表明，菌株YIM 77409t与t的再亲缘关系仅为30.6±1.6%，应视为新种的代表。scotoductusDSM 8553 t。根据形态学和化学分类特征以及基因型数据，提出菌株YIM 77409为热菌属的一个新种，命名为ethermus amyloliquefacienssp。11 .型应变为YIM 77409T（= DSM 25898T= KCTC 32024T）。
A Gram-stain-negative, aerobic bacterium, designated strain YIM 77409T, was isolated from the Niujie hot spring in the Eryuan county of Dali, Yunnan province, south-west China. Cells of the strain were rod-shaped and colonies were yellow and circular. The strain grew at pH 6.0–8.0 (optimum, pH 7.0) and 50–70°C (optimum, 60–65°C). The predominant menaquinone was MK-8 and the DNA G+C content was 66.4 mol%. Major fatty acids (>10 %) were iso-C15 : 0and iso-C17 : 0.The polar lipids consisted of one aminophospholipid, one phospholipid and two glycolipids. 16S rRNA gene sequence analysis showed that strain YIM 77409Tformed a cluster withThermus scotoductusDSM 8553T,Thermus antranikianiiDSM 12462T,Thermus caliditerraeYIM 77925TandThermus tengchongensisYIM 77924T, with highest 16S rRNA gene sequence similarity toT. scotoductusDSM 8553T(97.57 %). However, DNA–DNA hybridization indicated that strain YIM 77409Tshould be viewed as a representative of a novel species, as there was only 30.6 ± 1.6 % reassociation withT. scotoductusDSM 8553T. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain YIM 77409Trepresents a novel species of the genusThermus, with the nameThermus amyloliquefacienssp. nov. The type strain is YIM 77409T( = DSM 25898T= KCTC 32024T).