Investigation into the possible natural occurence of semicarbazide in Macrobrachium rosenbergii prawns.
Investigation into the possible natural occurence of semicarbazide in Macrobrachium rosenbergii prawns.
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DOI：
10.1021/jf103282g
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发表时间：
2011-02
影响因子：
6.1
通讯作者：
C. Van Poucke;Christ'l M. Detavernier;M. Wille;J. Kwakman;P. Sorgeloos;C. V. Van Peteghem
中科院分区：
文献类型：
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作者：
C. Van Poucke;Christ'l M. Detavernier;M. Wille;J. Kwakman;P. Sorgeloos;C. V. Van Peteghem
在过去的一年里，比利时进口淡水罗氏沼虾中氨基脲（SEM）检测呈阳性的病例有所增加，似乎表明可能滥用了一种被禁止的抗菌剂呋喃唑酮。这与所有其他欧洲国家形成鲜明对比，在这些国家，检测到SEM阳性样本的数量没有显著增加。比利时和其他欧盟成员国之间存在这种差异的一个可能解释是，仅在比利时分析了整只对虾（肉+壳）中是否存在硝基呋喃的组织结合代谢物，而在其他国家，仅分析了这些对虾的可食用部分（肉）。为了研究淡水对虾中SEM的可能自然发生，建立了动物试验。本实验分两组，每组10只幼鱼。将先前在标准化实验室条件下饲养的罗氏沼虾饲养在两个单独的水族箱中，一个对照组在参考条件下（不添加呋喃西林），另一个组暴露于每日剂量为50 mg的呋喃西林L（-1）的培养水。动物实验结果证明，扫描电镜自然发生在M。但在当前最低要求性能限值（MRPL）下，未处理动物的肉中未发现组织结合SEM。除了该动物试验之外，还分析了其他甲壳类动物物种的商业样品（其壳和肉分别进行了分析）是否存在SEM。
In the past year there has been an increased incidence in Belgium of cases of positive semicarbazide (SEM) tests in imported freshwater Macrobrachium rosenbergii prawns, seemingly indicating the possible abuse of nitrofurazone, a banned antimicrobial agent. This was in contrast to all other European countries where no significant increase in SEM-positive samples was detected. A possible explanation for this discrepancy between Belgium and the other European Union member states could be the fact that only in Belgium were whole prawns (meat + shell) analyzed for the presence of tissue-bound metabolites of nitrofurans, whereas in the other countries only the edible part (meat) of these prawns was analyzed. To investigate the possible natural occurrence of SEM in freshwater prawns, an animal trial was set up. In this experiment two groups of 10 juvenile M. rosenbergii, previously raised under standardized laboratory conditions, were stocked into two separate aquaria, a control group under reference conditions (no addition of nitrofurazone) and a group exposed to a daily dose of 50 mg of nitrofurazone L(-1) of culture water. Results of this animal trial proved that SEM naturally occurs in M. rosenbergii prawns but that at the current minimum required performance limit (MRPL) no tissue-bound SEM can be found in the meat of nontreated animals. In addition to this animal trial, commercial samples of other crustacean species, the shell and meat of which were analyzed separately, were also analyzed for the presence of SEM.