Fluorescein applications as fluorescent probes for the detection of analytes
Fluorescein applications as fluorescent probes for the detection of analytes
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DOI：
10.1016/j.trac.2017.08.013
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Fanyong Yan;Keqing Fan;Zhangjun Bai;Ruiqi Zhang;Fanlin Zu;Jinxia Xu;Li Xiang
中科院分区：
文献类型：
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作者：
Fanyong Yan;Keqing Fan;Zhangjun Bai;Ruiqi Zhang;Fanlin Zu;Jinxia Xu;Li Xiang
黄绿素衍生物是重要的荧光探针，可用于检测和光学成像。荧光素衍生物通常通过在荧光素氧杂蒽环和苯部分上引入醛基或酯化来构建。通常，将氨基与荧光素单醛连接的研究方向是热门。由于它们的高活性，这些衍生物可以与分析物络合以产生颜色的变化和荧光强度的增加或减少。本文根据荧光素探针的分析对象，包括金属离子、阴离子、小分子和生物大分子，对近两年来荧光素探针的研究进展进行了综述。本文综述了荧光素探针的合成方法、光学性质、可能的作用机理及其应用。本文为高灵敏度荧光素探针的筛选和生物检测提供了参考，并将推动其在分析物传感和检测中的进一步应用。
Fluorescein derivatives are important fluorescent probes which can be used for detection and optical imaging. Fluorescein derivatives are usually constructed by introducing aldehyde groups or esterified onto fluorescein xanthene ring and benzene moiety. Typically, the research direction of connecting amino groups with fluorescein monoaldehyde is in hot. Because of their high activity, these derivatives can be complexed with the analytes to produce changes of colors and the increase or decrease of fluorescence intensity. This article reviewed fluorescein probes in the past two years according to classification of different analytes including metal ions, anions, small molecules and biological macromolecules. The synthesis methods, optical properties, possible mechanisms and applications of fluorescein probes are summarized. This article provides a reference for the screening of fluorescein probes with high sensitivity and biological detection and can propel their further applications in sensing and detection of analytes.