Continuous high-dose antigen exposure preferentially induces IL-10, but intermittent antigen exposure induces IL-4.
Continuous high-dose antigen exposure preferentially induces IL-10, but intermittent antigen exposure induces IL-4.
复制标题
连续高剂量抗原暴露优先诱导IL-10，但间歇性抗原暴露诱导IL-4。
DOI：
10.1111/exd.12295
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Mizutani H.
中科院分区：
文献类型：
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作者：
Yamanaka K;Nakanishi T;Watanabe J;Kondo M;Yamagiwa A;Gabazza EC;Mizutani H.
IL-10在诱导特异性T细胞耐受中起关键作用。迄今为止，抗原应用诱导IL-10是否具有剂量或时间依赖性仍不清楚。在本研究中，在几种时间表中研究了过敏原暴露对IL-10的诱导作用。用恶唑酮反复作用于小鼠耳部，检测皮损中炎性细胞因子mRNA的表达。结果表明，连续高剂量抗原暴露诱导IL-4以及大量IL-10产生。单核细胞/树突状细胞和T细胞是IL-10的主要来源。过敏原特异性免疫治疗在抗原散射前恢复：季前赛。我们评估了季前治疗中过敏原的安全负荷剂量，重点是Tr 1诱导。用高剂量重新开始免疫治疗有效地增加了IL-10的表达，伴随着IL-4和炎性细胞因子的进一步诱导。因此，使用低剂量抗原重新开始的方案优先考虑加重或速发型过敏反应的风险。
IL‐10 plays a critical role in the induction of specific T‐cell tolerance. To date, whether IL‐10 induction by antigen application is dose‐ or time‐dependent remains unclear. In this study, IL‐10 induction by allergen exposure was investigated in the several schedules. Oxazolone was repeatedly applied to mouse ear, and mRNA of inflammatory cytokines in lesional skins was measured. The results indicated that continuous high‐dose antigen exposure induces IL‐4 as well as abundant IL‐10 production. Monocytes/dendritic cells and T cells are major source of IL‐10. Allergen‐specific immunotherapy is resumed before antigen scattering: preseason. We evaluated safe‐loading dose of allergens in preseasonal therapy focusing Tr1 induction. Restarting immunotherapy with high dose effectively augmented IL‐10 expression accompanied with further induction of IL‐4 and inflammatory cytokines. Therefore, the protocol restarting with low‐dose antigen is preferential to obviate the risk of exacerbation or anaphylaxis.