New method for the isolation and identification of methanogenic bacteria.
New method for the isolation and identification of methanogenic bacteria.
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产甲烷菌分离鉴定新方法
DOI：
10.1128/aem.29.4.540-545.1975
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
B. McBride
中科院分区：
文献类型：
--
作者：
T. Edwards;B. McBride
报道了一种利用培养皿快速生长和检测产甲烷细菌的新技术。该方法采用厌氧手套箱，该手套箱包含具有单独的气体冲洗设施的内室。从生活污泥中回收的产甲烷菌数量与其他方法回收的产甲烷菌数量相当。从污泥中分离的产甲烷生物包括甲烷八叠球菌属、甲烷八叠球菌属、甲烷杆菌属菌株M.o.H.和甲酸甲烷杆菌。通过利用这些生物体的独特荧光特性，促进了含有产甲烷细菌的菌落的鉴定。小至0.5 mm的菌落可以通过将其暴露于长波紫外线下来检测。
A new technique is reported for the rapid growth and detection of methanogenic bacteria by using petri plates. The method employs an anaerobic glove box containing an inner chamber with separate gas-flushing facilities. The numbers of methanogenic bacteria recovered from domestic sewage sludge are comparable to those recovered by other methods. The methanogenic organisms isolated from sludge include Methanosarcina, Methanospirillum, Methanobacterium strain M.o.H., and Methanobacterium formicicum. Identification of colonies containing methanogenic bacteria is facilitated by taking advantage of the unique fluorescence properties of these organisms. Colonies as small as 0.5 mm can be detected by exposing them to long-wave ultraviolet light.