Molecular cloning, genomic organization, and expression of a B-type (cricket-type) allatostatin preprohormone from Drosophila melanogaster
Molecular cloning, genomic organization, and expression of a B-type (cricket-type) allatostatin preprohormone from Drosophila melanogaster
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DOI：
10.1006/bbrc.2001.4402
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发表时间：
2001-02-23
影响因子：
3.1
通讯作者：
Grimmelikhuijzen, CJP
中科院分区：
文献类型：
--
作者：
Williamson, M;Lenz, C;Grimmelikhuijzen, CJP
昆虫的allatostatins之所以得名，是因为它们阻断了异化体（昆虫大脑附近的两个内分泌器官）中幼体激素（一种萜类）的生物合成。化学上，allatostatins可以细分为三个不同的肽群：a型allatostatins，首先在蟑螂中发现，其c端序列为Y/FXFGLamide；b型抑素，首先在蟋蟀中发现，其c端序列均为W(X)(6)Wamide；C型分配抑素，首先在Manduca sexta蛾中发现，它有一个不相关的、未经修饰的C端。我们之前报道过果蝇果蝇a型抑素前原激素的结构。本文描述了果蝇b型预抑素（DAP-B）的分子克隆。DAP-B全长211个氨基酸残基，含有以下假定的抑素他汀类药物：AWQSLQSSW-amide（抑素他汀- b1）， AWKSMNVAWamide（抑素他汀- b2），
The insect allatostatins obtained their names because they block the biosynthesis of juvenile hormone (a terpenoid) in the corpora allata (two endocrine organs near the insect brain). Chemically, the allatostatins can be subdivided into three different peptide groups: the A-type allatostatins, first discovered in cockroaches, which have the C-terminal sequence Y/FXFGLamide in common; the B-type allatostatins, first discovered in crickets, which all have the C-terminal sequence W(X)(6)Wamide; and the C-type allatostatins, first discovered in the moth Manduca sexta, which have an unrelated and nonamidated C terminus. We have previously reported the structure of an A-type allatostatin preprohormone from the fruitfly Drosophila melanogaster. Here we describe the molecular cloning of a B-type prepro-allatostatin from Drosophila (DAP-B). DAP-B is 211 amino acid residues long and contains one copy each of the following putative allatostatins: AWQSLQSSW-amide (drostatin-B1), AWKSMNVAWamide (drostatin-B2),