Homotypic vacuole fusion requires Sec17p (yeast alpha-SNAP) and Sec18p (yeast NSF)
Homotypic vacuole fusion requires Sec17p (yeast alpha-SNAP) and Sec18p (yeast NSF)
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DOI：
10.1002/j.1460-2075.1996.tb00694.x
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发表时间：
1996-07-01
期刊：
影响因子：
11.4
通讯作者：
Wickner, W
中科院分区：
文献类型：
--
作者：
Haas, A;Wickner, W
在酿酒酵母中，液泡是由母液泡形成管状和泡状结构，这些结构定向投射到芽中，以及这些囊泡的同型融合而遗传的。我们以前已经利用了一种无细胞遗传分析来表明，液泡遗传的融合步骤需要胞浆、ATP和GTP酶Ypt7p。在这里，我们使用亲和纯化的抗体和纯化的重组蛋白证明了在体外同型空泡融合中需要Sec17p(酵母α-SNAP)和Sec18p(酵母NSF)。因此，通常参与异型转运步骤的Sec17p和Sec18p也可以参与同型细胞器融合。我们进一步证明，某些脂肪酰辅酶A化合物以Sec18p依赖的方式刺激液泡到液泡的融合。最后，我们的数据表明存在一种胞浆因子，它可以激活液泡膜结合的Sec18p。
In Saccharomyces cerevisiae, vacuoles are inherited by the formation of tubular and vesicular structures from the mother vacuole, the directed projection of these structures into the bud and the homotypic fusion of these vesicles. We have previously exploited a cell-free inheritance assay to show that the fusion step of vacuole inheritance requires cytosol, ATP and the GTPase Ypt7p. Here we demonstrate, using affinity-purified antibodies and purified recombinant proteins, a requirement for Sec17p (yeast alpha-SNAP) and Sec18p (yeast NSF) in homotypic vacuole fusion in vitro. Thus, Sec17p and Sec18p, which are typically involved in heterotypic transport steps, can also be involved in homotypic organelle fusion. We further show that vacuole-to-vacuole fusion is stimulated by certain fatty acyl-coenzyme A compounds in a Sec18p-dependent fashion. Finally, our data suggest the presence of a cytosolic factor which activates vacuole membrane-bound Sec18p.