Characterization of Toxoplasma gondii isolates from free range chickens from Parana, Brazil
Characterization of Toxoplasma gondii isolates from free range chickens from Parana, Brazil
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DOI：
10.1016/j.vetpar.2003.09.003
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发表时间：
2003-11-14
影响因子：
2.6
通讯作者：
Lehmann, T
中科院分区：
文献类型：
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作者：
Dubey, JP;Navarro, IT;Lehmann, T
自由放养鸡中弓形虫的流行是环境中弓形虫卵囊流行的一个很好的指标，因为鸡是从地面进食的。在本研究中，对来自巴西巴拉那周围农村地区的40只散养鸡(Gallus Home Ticus)的弓形虫流行率进行了评估。对每只鸡的血液、心脏和大脑进行了弓形虫感染检查。用改良的弓形虫凝集试验(MAT大于或等于1：5)检测16只鸡的弓形虫抗体。用小鼠对血清阳性(MAT大于或等于1：5)鸡的心脏和脑进行了生物测定。此外，在两只没有弓形虫的猫(每只猫12只)中，对血清阴性(Mat<；1：5)鸡的心脏和大脑进行了生物检测。从16只血清阳性鸡中分离到13株弓形虫(81%)。在两只喂食血清阴性鸡组织的猫中，一只猫脱落了弓形虫卵囊。在13个弓形虫分离株中，有9个能100%杀死感染的小鼠。从猫身上分离出的弓形虫对小鼠也有毒力。用SAG2基因座对13个鸡弓形虫分离株进行基因分型，发现7个分离株为I型，6个为III型，其中3个III型分离株对所有感染的小鼠均有致死作用，表明所有对小鼠有毒力的分离株都不是I型，而从猫粪中分离的分离株为I型。
The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T. gondii oocysts in the environment because chickens feed from the ground. In the present study, prevalence of T. gondii in 40 free range chickens (Gallus domesticus) from a rural area surrounding Parana, Brazil was assessed. Blood, heart, and brain from each chicken were examined for T. gondii infection. Antibodies to T. gondii, assayed with the modified agglutination test (MAT greater than or equal to 1:5) were found in 16 chickens. Hearts and brains of seropositive (MAT greater than or equal to 1:5) chickens were bioassayed in mice. Additionally, hearts and brains of seronegative (MAT < 1:5) chickens were bioassayed in two T. gondii-free cats (12 chickens per cat). T. gondii was isolated from 13 of 16 (81%) seropositive chickens. Of the two cats fed tissues pooled form seronegative chickens, one shed T. gondii oocysts. Nine of the 13 T. gondii isolates killed 100% of infected mice. The T. gondii isolate from the cat was also virulent for mice. Genotyping of 13 chicken isolates of T. gondii using the SAG2 locus indicated that seven isolates were type I and six were type III; three of these type III isolates killed all infected mice suggesting that all strains virulent for mice are not type I. The isolate from the feces of the cat fed chicken tissues was type I. Published by Elsevier B.V.